Bacterial cell factories for the production of industrial relevant biomolecules
Many bacteria secrete proteins, mainly enzymes, into the extracellular space. Effective secretion significantly facilitates down-stream processing and thus represents a major prerequisite for biotechnological protein production. Major aims of our working group are the understanding and biotechnological application of the secretion mechanisms.
Specifically, we study protein production and secretion in the Gram-negative bacterium Burkholderia glumae which is used by the company BASF SE as a lipase production strain, and in the Gram-positive bacterium Bacillus subtilis which is able to secrete large amounts of protein into the growth medium and is therefore of great interest for biotechnological applications.
Our methods include an automated pipetting robot and fluorescence detection devices enabling high-throughput library screening systems based on enzyme activity or fluorescence labeled proteins. First scale-ups are also possible using table-top fermentation systems with up to on liter of cultivation volume.
- Production of biomass degrading enzymes in B. subtilis and E. coli (funded by Bioeconomy Science Center (Projekt BioBreak and Junior Research Group)
- Secretion of lipase by B. glumae (funded by BMBF, Projekt MiPro)
- Role of signal sequence for secretion of a lipase by B. subtilis (funded by CLIB2021 Graduate Cluster)
- Comprehensive site-saturation mutagenesis to study the role of each amino acid for stability and secretion of B. subtilis lipase (DFG RTG BioNoCo)