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Publications – Kovacic Filip - May 2023




Gentile R, Modric M, Thiele B, Jaeger K-E, Kovacic F, Gohlke H

Molecular mechanisms underlying medium-chain length free fatty acid-regulated activity of the phospholipase PlaF from P. aeruginosa [Link]

bioRxiv (2023) DOI: doi.org/10.1101/2023.05.02.539057


Bandyopadhaya A, Singh V, Chakraborty A, Kovacic F, Oldham WM, Tzika AA, Wilk SH, Rahme LG

Bacterial quorum-sensing signal rewires immune cell metabolism to mediate tolerance to infection by impeding cellular bioenergetics

PNAS (2023) Submitted.


Hofmann D, Thiehle B, Rahmati M, Siebers M, Ventura M, Schütz V, Jeong S, Bigler L, Held F, Wu B, Babic N, Kovacic F, Hamacher J, Hölzl G, Dörmann P, Schulz M

A below ground chemical fight for phosphate and habitat - interactions of Camelina sativa (L.) Crantz with microorganisms [Link]

Submitted (2023) DOI: 10.21203/rs.3.rs-2247394/v1


Caliskan M, Poschmann G, Gudzuhn M, Strunk CH, Molitor R, Waldera-Lupa D, Streit WR, Jaeger K-J, Stühler K and Kovacic F

Virulence adaptation of Pseudomonas aeruginosa phospholipase mutant with altered membrane phospholipid composition [Link]

Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids (2023) 1868:159317.


Dittrich J, Popara M, Kubiak J, Dimura M, Schepers B, Verma N, Schmitz B, Dollinger P, Kovacic F, Jaeger K-E, Seidel C, Peulen T-O, Gohlke H

Resolution of maximum entropy method-derived posterior conformational ensembles of a flexible system probed by FRET and molecular dynamics simulations [Link]

Journal of Chemical Theory and Computation (2023) 19:2409.




Papadopoulos A, Busch M, Reiners J, Hachani E, Baeumers M, Schmitt L, Jaeger K-E, Kovacic F, Smits HJS, Kedrov A

The periplasmic chaperone Skp prevents misfolding of the secretory lipase A from Pseudomonas aeruginosa [Link]

Frontiers in Molecular Biosciences (2022) 9:1026724.


Bleffert F, Granzin J, Caliskan M, Schott-Verdugo SN, Siebers M, Thiele B, Rahme L, Dörmann P, Gohlke H, Batra-Safferling R, Jaeger K-E and Kovacic F

Structural, mechanistic and physiological insights into phospholipase A-mediated membrane phospholipid degradation in Pseudomonas aeruginosa [Link]

eLife (2022) 11:e72824


Weiler AJ, Spitz O, Gudzuhn M, Schott-Verdugo SN, Kamel M, Thiele B, Streit WR, Kedrov A, Schmitt L, Gohlke H and Kovacic F

A phospholipase B from Pseudomonas aeruginosa with activity towards endogenous phospholipids affects biofilm assembly [Link]

Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids (2022) 4: 159101.






Ahmad S, Strunk CH, Schott-Verdugo SN, Jaeger K-E, Kovacic F and Gohlke H

Substrate access mechanism in a novel membrane-bound phospholipase A of Pseudomonas aeruginosa concordant with specificity and regioselectivity [Link]

Journal of Chemical Information and Modeling (2021) 61: 5626 – 5643.


Babic N and Kovacic F

Predicting drug targets by homology modelling of Pseudomonas aeruginosa proteins of unknown function [Link]

PLoS ONE (2021) 16: e0258385.


Perez-Garcia P, Kobus S, Hoeppner A, Holzscheck N, Strunk CH, N. N., Huber NNH, Jaeger K-E, Kovacic F, Smits S, Streit WR, Chow J

The origin of metallo-beta-lactamases: The highly promiscuous hydrolase Igni18 from the crenarchaeon Ignicoccus hospitalis represents the paradigm of an ancestral enzyme [Link]

Communications Biology (2021) 4: 132.



Viegas A, Dollinger P, Verma N, Kubiak J, Viennet T, Seidel CAM, Gohlke H, Etzkorn M, Kovacic F, and Jaeger K-E

Structural and dynamic insights revealing how lipase binding domain MD1 of Pseudomonas aeruginosa foldase affects lipase activation  [Link]

Scientific Reports (2020) 10: 3578.




Verma N, Dollinger P, Kovacic F, Jaeger K-E, Holger G.

The membrane-integrated steric chaperone Lif facilitates active site opening of Pseudomonas aeruginosa lipase A [Link]

Journal of Computational Chemistry (2019) 9999: 1 – 13.


Bleffert F, Granzin J, Gohlke H, Batra-Safferling R, Jaeger K-E, Kovacic F

Pseudomonas aeruginosa esterase PA2949, a bacterial homolog of the human membrane esterase ABHD6: expression, purification and crystallization [Link]

Acta Crystallogr F Struct Biol Commun (2019) F75: 270-277.


Kobus S, Perez-Garcia P, Hoeppner A, Holzcheck N, Streit W, Kovacic F, Jaeger K-E, Chow J and Smits S

Igni18, a novel metallo-hydrolase from the hyperthermophilic archaeon Ignicoccus hospitalis KIN4/I: cloning, expression, purification and X-ray analysis [Link]

Acta Crystallogr F Struct Biol Commun (2019) F75: 307-311.


Wanga Y, Horlamus F, Kovacic F, Kölling-Paternoga R, Henkel M, Hausmann R, Wittgens W, Rosenau F.

Growth of engineered Pseudomonas putida KT2440 on glucose, xylose and arabinose: hemicellulose hydrolysates and their major sugars as sustainable carbon sources [Link]

GCB Bioenergy (2019) 11: 249-259.




Bücher K S, Babic N, Freichel T, Kovacic F and Hartmann L

Monodisperse sequence-controlled α-L-fucosylated glycooligomers and their multivalent inhibitory effects on LecB   [Link]

Macromolecular Biosciences (2018) e1800337.


Kovacic F., Babić N., Krauss U. and Jaeger K.-E.

Classification of Lipolytic Enzymes from Bacteria [Link]

In Handbook of Hydrocarbon and Lipid Microbiology, K. Timmis, Editor. Springer Berlin Heidelberg. (2018) 1-35. 


Jaeger K-E, Fulton A, Schwaneberg U, Pietruszka J, Kovacic F.

Identification of stable and enantioselective lipases for biotechnological applications [Link]

Croatica Chemica Acta (2018) 91: 249-253.


Fulton A, Kovacic F, Schwaneberg U, Pietruszka J, Jaeger K-E.

Bestimmung der Stabilität und Enantioselektivität von Lipasen [Link]
Biospektrum (2018) 24: 156-159.


Siebers M, Rohr T, Ventura M, Thies S, Kovacic F, Jaeger K-E, Berg M, Doermann P, Schulz M.

Disruption of Microbial Community Composition and Enrichment with Plant Growth Promoting Microorganisms after Exposure of Soil to Rapeseed-Derived Glucosinolates      [Link]

PlosONE (2018) 13: e0200160.




Wittgens A, Kovacic F, Müller MM, Gerlitzki M, Santiago-Schübel B, Hofmann D, Henkel M, Hausmann R, Wilhelm S, Rosenau F.

Novel insights into the biosynthesis and the uptake of rhamnolipids and their precursors.    [Link]

Applied Microbiology and Biotechology (2017) 10: 2865-2878.




Classen T, Kovacic F, Lauinger B, Pietruszka J, Jaeger K-E.

Screening for Enantioselective Lipases.   [Link]

In book: Hydrocarbon and Lipid Microbiology Protocols. Eds. Terry J. McGenity, Kenneth N. Timmis and Balbina Nogales. Springer-Verlag (2016) 1-33.


 Thies S, Rausch S C, Kovacic F, Schmidt-Thaler A, Wilhelm S, Rosenau F, Daniel R, Streit W, Pietruszka J, Jaeger K-E.

Metagenomic discovery of novel enzymes and biosurfactants in a slaughterhouse biofilm microbial community.  [Link]

Sci Rep (2016) 6: 27035.


Kovacic F, Bleffert F, Caliskan M, Wilhelm S, Granzin J, Batra-Safferling R, Jaeger K-E.

A membrane-bound esterase PA2949 from Pseudomonas aeruginosa is expressed and purified from Escherichia coli.    [Link]

FEBS Open Bio (2016) 6, 484-493.  




Kovacic F, Mandrysch A, Poojari C, Strodel B, Jaeger K-E.

Structural features determining thermal adaptation of esterases .    [Link]

Protein Eng Des Sel (2015) 29: 65-76.


Tchigvintsev A, Tran H, Popovic A, Kovacic F, Brown G, Flick R, Hajighasemi M, Egorova O, Somody JC, Tchigvintsev D, Khusnutdinova A, Chernikova TN, Golyshina OV, Yakimov MM, Savchenko A, Golyshin PN, Jaeger K-E, Yakunin AF.

The environment shapes microbial enzymes: five cold-active and salt-resistant carboxylesterases from marine metagenomes.    [Link]

Appl Microbiol Biotechnol (2015) 99: 2165-2178.




Drepper T, Funken H, Heck A, Kovacic F, Krauss U, Pietruszka J.

Special Issue dedicated to Karl-Erich Jaeger on the occasion of his 60th birthday.    [Link]

J Biotechnol (2014) 191: 1-2.


Thies S, Santiago-Schübel B, Kovacic F, Rosenau F, Hausmann R, Jaeger K-E.

Heterologous production of the lipopeptide biosurfactant serrawettin W1 in Escherichia coli.    [Link]

J Biotechnol (2014) 181:27-30.


Jaeger K-E and Kovacic F.

Determination of lipolytic enzyme activities.    [Link]

Methods Mol Biol (2014) 1149: 111-134.


Buerth C, Kovacic F, Stock J, Terfrüchte M, Wilhelm S, Jaeger K-E, Feldbruegge M, Schipper K, Ernst JF, Tielker D.

Uml2 is a novel CalB-type lipase of Ustilago maydis with phospholipase A activity.    [Link]

Appl Microbiol Biotechnol (2014) 98: 4963-4973.




Kovačić F, Granzin J, Wilhelm S, Kojić-Prodić B, Batra-Safferling R, Jaeger K-E.

Structural and functional characterisation of TesA - a novel lysophospholipase A from Pseudomonas aeruginosa.    [Link]

PLoS One (2013) 8: e69125.


Ašler IL, Kovačić F, Marchetti-Deschmann M, Allmaier G, Stefanić Z, Kojić-Prodić B.

Inhibition of extracellular lipase from Streptomyces rimosus with 3,4-dichloroisocoumarin.    [Link]

J Enzyme Inhib Med Chem (2013) 28: 1094-1104.




Chow J, Kovačić F, Dall Antonia Y, Krauss U, Fersini F, Schmeisser C, Lauinger B, Bongen P, Pietruszka J, Schmidt M, Menyes I, Bornscheuer UT, Eckstein M, Thum O, Liese A, Mueller-Dieckmann J, Jaeger K-E and Streit WR.

The metagenome-derived enzymes LipS and LipT increase the diversity of known lipases.    [Link]

PLoS ONE (2012) 7: e47665.


Perez D, Kovacic F, Wilhelm S, Jaeger K-E, Garcia MT, Ventosa A, Mellado E.

Identification of amino acids involved in hydrolytic activity of lipase LipBL from Marinobacter lipolyticus.    [Link]

Microbiology (2012) 158: 2192-2203.




Salacha R, Kovacic F, Brochier-Armanet C, Wilhelm S, Tommassen J, Filox A, Voulhoux R, Bleves S.

The Pseudomonas aeruginosa patatin-like protein PlpD is the archetype of a novel Type V secretion system.    [Link]

Environ Microbiol (2010) 12: 1498-1512.


Lescic Asler I, Ivic N, Kovacic F, Schell S, Knorr J, Krauss U, Wilhelm S, Kojic-Prodic B, Jaeger K. E.

Probing enzyme promiscuity of SGNH hydrolases.    [Link]

Chembiochem (2010) 11: 2158-2167.




Lescic Asler I, Zehl M, Kovacic F, Muller R, Abramic M, Almaier G, Kojic-Prodic B.

Mass spectrometric evidence of covalently-bound tetrahydrolipstatin at the catalytic serine of Streptomyces rimosus lipase.    [Link]

Biochim Biophys Acta (2007) 1770: 163-170.