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Troost K., Loeschcke A., Hilgers F., Özgür A., Weber T.M., Santiago-Schübel B., Svensson V., Hage-Hülsmann J., Habash S., Grundler F.M.W., Schleker S., Jaeger K.-E., Drepper T. (2019): Engineered Rhodobacter capsulatus as a phototrophic platform organism for the synthesis of plant sesquiterpenoids. Front Nutr, accepted, doi: 10.3389/fmicb.2019.01998.

Otto M., Wynands B., Drepper T., Jaeger K.-E., Thies S., Loeschcke A., Blank L.M., Wierckx N.  (2019): Targeting 16S rDNA for stable recombinant gene expression in Pseudomonas. ACS Synth Biol 8: 1901-1912, doi: 10.1021/acssynbio.9b00195.

Weihmann R., Domröse A., Drepper T., Jaeger K.-E., Loeschcke A. (2019): Protocols for yTREX/Tn5-based gene cluster expression in Pseudomonas putida. Microb Biotechnol 0: 1-13, doi: 10.1111/1751-7915.13402.

Domröse A., Hage-Hülsmann J., Thies S., Weihmann R., Kruse L., Otto M., Wierckx N.,Jaeger K.-E., Drepper T., Loeschcke A. (2019): Pseudomonas putida rDNA as an exceptionally suitable site for the expression of biosynthetic genes. Sci Rep 9: 7028, doi: 10.1038/s41598-019-43405-1.


Kallscheuer N., Classen T., Drepper T., Marienhagen J (2019): Production of plant metabolites with applications in the food industry using engineered microorganisms. Curr Opin Biotechnol 56: 7-17, doi: 10.1016/j.copbio.2018.07.008.


Peters L., Weidenfeld I., Klemm U., Loeschcke A., Weihmann R., Jaeger K.-E., Drepper T., Ntziachristos V., Stiel A.C. (2019): Phototrophic purple bacteria as optoacoustic in vivo reporters of macrophage activity. Nat Comm 10: 1191, doi: 10.1038/s41467-019-09081-5.


Coscolín C., Katzke N., García-Moyano A., Navarro-Fernández J., Almendral D., Martínez-Martínez M., Bollinger A., Bargiela R., Gerler C., Chernikova T., Rojo D., Barbas C, Hai T, Golyshina O, Koch R, Yakimov M, Bjerga G, Golyshin P, Jaeger K.-E., and Ferrer M. (2018): Bioprospecting reveals class III ω-transaminases converting bulky ketones and environmentally relevant polyamines. Appl Environ Microbiol 85: e02404-18, doi: 10.1128/AEM.02404-18.


Burmeister A., Hilgers F., Rudat A., Westerwalbesloh C., Kerkhoff Y., Drepper T., Kohlheyer D., Noack S., Grünberger A. (2019): A microfluidic co-cultivation platform to investigate interactions in microbial communities on single-cell level. Lab Chip 19: 98, doi: 10.1039/c8lc00977e.


Endres S., Wingen M., Torra J., Ruiz-González R., Polen T., Bosio G., Bitzenhofer N.L., Hilgers F., Gensch T., Nonell S., Jaeger K.-E., Drepper T. (2018): An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria. Sci Rep 8: 15021, doi: 10.1038/s41598-018-33291-4.

Streit W., Chow J., Jaeger K.-E., Katzke N., Koch R., Borchert M., Bjørnvad M., Roggenbuck M., Lippold F., Golyshin P., Golyshina O., Tran H., Ferrer M., Daniel R., Hollensteiner J. (2018): A metagenomic collection of novel and highly efficient biocatalysts for industrial biotechnology. Impact, 82–84, doi: 10.21820/23987073.2018.5.82.


Hage-Hülsmann J., Grünberger A., Thies S., Santiago-Schübel B., Klein A.S., Pietruszka J., Binder D., Hilgers F., Domröse A., Drepper T., Kohlheyer D., Jaeger K.-E., Loeschcke A. (2018): Natural biocide cocktails: combinatorial antibiotic effects of prodigiosin and biosurfactants. PLoS ONE 13: e0200940, doi: 10.1371/journal.pone.0200940.


Fettweiss T., Röllen K., Granzin J., Reiners O., Endres S., Drepper T., Willbold D., Jaeger K.-E., Batra-Safferling R., Krauss U. (2018): Mechanistic basis of the fast dark recovery of the short LOV protein DsLOV from Dinoroseobacter shibae. Biochemistry 57: 4833-4847, doi: 10.1021/acs.biochem.8b00645.

Bollinger A., Thies S. Katzke N., Jaeger K.-E. (2018): The biotechnological potential of marine bacteria in the novel lineage of Pseudomonas pertucinogena. Microb Biotechnol 0: 1-13, doi: 10.1111/1751-7915.13288.


Klein A.S., Brass H.U.C., Klebl D.P., Classen T., Loeschcke A., Drepper T., Sievers S., Jaeger K.-E., Pietruszka J. (2018): Preparation of cyclic prodiginines by mutasynthesis in Pseudomonas putida KT2440. Chembiochem 19: 1545-1552, doi: 10.1002/cbic.201800154.


Loeschcke A., Dienst D., Wewer V., Hage-Hülsmann J., Dietsch M., Kranz-Finger S., Hüren V., Metzger S., Urlacher V.B., Gigolashvili T., Kopriva S., Axmann I.M., Drepper T., Jaeger K.-E. (2017): The photosynthetic bacteria Rhodobacter capsulatus and Synechocystis sp. PCC 6803 as new hosts for cyclic plant triterpene biosynthesis. PLoS ONE 12: e0189816, doi: 10.1371/journal.pone.0189816.

Domröse A., Weihmann R., Thies S., Jaeger K.-E., Drepper T., Loeschcke A. (2017): Rapid generation of recombinant Pseudomonas putida secondary metabolite producers using yTREX. Synth Syst Biotechno 2: 310-319, doi: 10.1016/j.synbio.2017.11.001.

Poehlein A., Daniel R., Thürmer A., Bollinger A., Thies S., Katzke N., Jaeger K.-E. (2017): First insights into the genome sequence of Pseudomonas oleovorans DSM 1045. Genome Annouc 5: e00774-17, doi: 10.1128/genomeA.00774-17.

Binder D., Drepper T., Jaeger K.-E., Delvigne F., Wiechert W., Kohlheyer D., Grünberger A. (2017): Homogenizing bacterial cell factories: analysis and engineering of phenotypic heterogeneity. Metab Eng 42: 145-156, doi: 10.1016/j.ymben.2017.06.009.

Klein A.S., Domröse S., Bongen P., Brass H.U.C., Classen T.,  Loeschcke A., Drepper T., Laraia L., Sievers S., Jaeger K.-E., Pietruszka J. (2017): New Prodigiosin derivatives obtained by mutasynthesis in Pseudomonas putida. ACS Synth Biol 6: 1757-1765, doi: 10.1021/acssynbio.7b00099.

Rupprecht C., Wingen M., Potzkei J., Gensch T. Jaeger K.-E., Drepper T. (2017): A novel FbFP-based biosensor toolbox for sensitive in vivo determination of intracellular pH. J Biotechnol 258: 25-32, doi: 10.1016/j.jbiotec.2017.05.006.

Heck A. & Drepper T. (2017): Engineering Photosynthetic α-Proteobacteria for the Production of Recombinant Proteins and Terpenoids. In Modern topics in the phototrophic prokaryotes – Environmental and applied aspects. Edited by P. C. Hallenbeck, 395-425. Cham/CH, Springer International Publishing, doi: 10.1007/978-3-319-46261-5_12.

Wingen M., Jaeger K.-E., Gensch T., Drepper T. (2017): Novel thermostable flavin-binding fluorescent proteins from thermophilic organisms. Photochem Photobiol 93: 849-856, doi: 10.1111/php.12740.

Katzke N., Knapp A., Loeschcke A., Drepper T., Jaeger K.-E. (2017): Novel Tools for the Functional Expression of Metagenomic DNA. In Metagenomics: Methods and protocols. 2nd edn, edited by W. R. Streit & R. Daniel. Methods Mol Biol 1539: 159-196. New York/USA, Humana Press, doi: 10.1007/978-1-4939-6691-2_10.

Bier C., Binder D., Drobietz D., Loeschcke A., Drepper T., Jaeger K.-E., Pietruszka J. (2017): Photo-caged carbohydrates – versatile tools for controlling gene expression by light. Synthesis 49: 42-52, doi: 10.1055/s-0035-1562617.

2016 Binder, D., Probst, C., Grünberger, A., Hilgers, F., Loeschcke, A., Jaeger, K.-E., Kohlheyer, D., Drepper, T. (2016). Comparative single-cell analysis of different E. coli expression systems during microfluidic cultivation. PLoS ONE 11: e0160711.
Binder, D., Frohwitter, J., Mahr, R., Bier, C., Grünberger, A., Loeschcke, A., Peters-Wendisch, P., Kohlheyer, D., Pietruszka, J., Frunzke, J., Jaeger, K.-E., Wendisch, V.F., Drepper, T. (2016). Light-controlled cell factories - Employing photocaged IPTG for light-mediated optimization of lac-based gene expression and (+)-valencene biosynthesis in Corynebacterium glutamicum. Appl Environ Microbiol. AEM.01457-16. [Epub ahead of print]
Binder, D., Bier, C., Grünberger, A., Drobietz, D., Hage-Hülsmann, J., Kohlheyer, D., Loeschcke, A., Wiechert, W., Jaeger, K.-E., Pietruszka, J., Drepper, T. (2016). Photocaged Arabinose – A Novel Optogenetic Switch for Rapid and Gradual Control of Microbial Gene Expression. Chem. Bio. Chem. 17: 296-299 doi: 10.1002/cbic.201500609
Davari, M.D., Kopka, B., Wingen, M., Bocola, M., Drepper, T., Jaeger, K.-E., Schwaneberg, U., Krauss, U. (2016). Photophysics of the LOV-based fluorescent protein variant iLOVQ489K determined by simulation and experiment. J. Phys. Chem. B, 120:3344-3352. doi: 10.1021

Katzke N., Knapp A., Loeschcke A., Drepper T., Jaeger K.-E. (2017). Novel Tools for the Functional Expression of Metagenomic DNA. Methods Mol. Biol. 1539:159-196

Shah, R., Pathak, G.P., Drepper, T., Gärtner, W. (2016). Selective photoreceptor gene knock-out reveals a regulatory role for the growth behavior of Pseudomonas syringae. Photochem Photobiol. 92:571-578. doi: 10.1111

Wandrey G., Bier C., Binder D., Hoffmann K., Jaeger K.-E., Pietruszka J., Drepper T., Büchs J. (2016). Light-induced gene expression with photocaged IPTG for induction profiling in a high-throughput screening system. Microb. Cell Fact. 15:63


Domröse, A., Klein, A., Hage-Hülsmann, J., Thies, S., Svensson, V., Classen, T., Pietruszka, J., Jaeger, K.-E., Drepper, T., Loeschcke, A. (2015). Efficient recombinant production of prodigiosin in Pseudomonas putida. Front. Microbiol. 6:972

Binder, D., Bier, C., Grünberger, A., Drobietz, D., Hage-Hülsmann, J., Kohlheyer, D., Loeschcke, A., Wiechert, W., Jaeger, K.-E., Pietruszka, J., Drepper, T. (2015). Photocaged Arabinose – A Novel Optogenetic Switch for Rapid and Gradual Control of Microbial Gene Expression. Chem. Bio. Chem. doi: 10.1002/cbic.201500609
Loeschcke, A, Thies, S. (2015). Pseudomonas putida - a versatile host for the production of natural products. Appl. Microbiol. Biotechnol. 99:6197-6214.
Endres, S., Granzin, J., Circolone, F., Stadler, A., Krauss, U., Drepper, T., Svensson, V., Knieps-Grünhagen, E., Wirtz, A., Cousin, A., Tielen, P., Willbold, D., Jaeger, K.-E., Batra-Safferling, R. (2015). Structure and function of a short LOV protein from the marine phototrophic bacterium Dinoroseobacter shibae. BMC Microbiol., 15:30.

Torra, J., Burgos-Caminal, A., Endres, S., Wingen, M., Drepper, T., Gensch, T., Ruiz-González, R., and Nonell, S. (2015). Singlet oxygen photosensitisation by the fluorescent protein Pp2FbFP L30M, a novel derivative of Pseudomonas putida flavin-binding Pp2FbFP. Photochem. Photobiol. Sci. 14:280-287


Liebl, W., Angelov, A., Juergensen, J., Chow, J., Loeschcke, A., Drepper, T., Classen, T., Pietruszka, J., Ehrenreich, A., Streit, W.R., Jaeger, K.-E. (2014). Alternative hosts for functional (meta)genome analysis. Appl. Microbiol. Biotechnol. 98: 8099-8109.

Binder, D., Grünberger, A., Loeschcke, A. Probst, C., Pietruszka, J., Wiechert, W., Kohlheyer, D., Jaeger, K.-E., and Drepper, T. (2014). Light-responsive control of bacterial gene expression: Precise triggering of the lac promoter activity using photocaged IPTG. Integr. Biol., 6: 755- 765

Kaschner, M., Loeschcke, A., Krause, J., Minhc, B. Q., Heck, A. Svensson, V., Wirtz, A., von Haeseler, A., Jaeger, K.-E., Drepper, T., and Krauss, U. (2014). The first light-dependent protochlorophyllide oxidoreductase in anoxygenic phototrophic bacteria. Molecular Microbiology. 93: 1066-1078

Wingen, M., Potzkei, J., Endres, S., Casini, G., Rupprecht, C., Krauss, U., Jaeger, K.-E., Drepper, T., Gensch, T. (2014). The photophysics of LOV-based fluorescent proteins – new tools for cell biology. Photochem. Photobiol. Sci., 13: 875-883


Drepper, T., Gensch, T. and Pohl, M. (2013). Advanced in vivo applications of blue light photoreceptors as alternative fluorescent proteins. Photochem. Photobiol. Sci., 12: 1125-1134


Loeschcke A., Markert A., Wilhelm S., Wirtz A., Rosenau F., Jaeger K.-E., and Drepper T. (2013). TREX – A Universal Tool for the Transfer and Expression of Biosynthetic Pathways in Bacteria. ACS Synth. Biol. 2, 22–33


Walter, J., Hausmann, S., Drepper, T., Puls, M., Eggert, T., and Dihné, M. (2012). Flavin mononucleotide-based fluorescent proteins function in mammalian cells without oxygen requirement. PLoS One, 7:e43921


Heck, A., Tielker, D., Ernst, J. F., Freudl, R., Bott, M., Oldiges, M., Wiechert, W., Pietruszka, J., Wilhelm, S., Rosenau, F., Drepper, T. & Jaeger, K.-E. (2012): Forschungsplattform ExpressO: Expressionsoptimierung in Mikroorganismen. BIOspektrum 18, 449-451.


Potzkei, J., Kunze, M., Drepper, T., Gensch, T., Jaeger, K.-E., Büchs, J (2012). Real-time determination of intracellular oxygen using a genetically encoded FRET-based biosensor. BMC Biology, 10:28


Circolone, F., Granzin, J., Jentzsch, K., Drepper, T., Jaeger, K.-E., Willbold, D., Krauss, U. and Batra-Safferling, R. (2012). Structural basis for the slow dark recovery of a full-length LOV protein from Pseudomonas putida. JMB. 417: 362–374


Arvani, S., Markert, A., Loeschcke, A., Jaeger, K.-E., and T. Drepper (2012). A T7 RNA polymerase-based toolkit for the concerted expression of clustered genes. J. Biotechnol. 159:162-171


Katzke, N., Jaeger, K.-E., and Drepper, T. (2012). High-level gene expression in the photosynthetic bacterium Rhodobacter capsulatus. Methods Mol. Biol. 824:251-269


Drepper, T., Krauss, U., Meyer zu Berstenhorst, S., Pietruszka, J., and Jaeger, K.-E. (2011).

Lights on and action! - How to control microbial gene expression by light. Appl. Microbiol. Biotechnol. 90: 23-40


Krauss U., Drepper T., Jaeger K.-E. (2011). Enlightened enzymes: Strategies to create novel photoresponsive proteins. Chem. Eur. J. 17(9):2552-2560


Troeschel, S. C., Drepper, T., Leggewie, C., Streit, W., and Jaeger, K.-E. (2010). Novel tools for the functional expression of metagenomic DNA. Methods Mol. Biol. 668:117-139.


Drepper, T., Huber, R., Heck, A., Circolone, F., Hillmer, A.-K., Büchs, J., and Jaeger K.-E. (2010). Flavin mononucleotide-based fluorescent reporter proteins outperform green fluorescent protein-like proteins as quantitative in vivo real-time reporters. Appl. Environ. Microbiol. 76: 5990–5994


Wagner-Döbler, I., Ballhausen, B., Berger, M., Brinkhoff, T., Buchholz, I., Bunk, B., Cypionka, H., Daniel, R., Drepper, T., Gerdts, G., Hahnke, S., Han, C., Jahn, D., Kalhoefer, D., Kiss, H., Klenk, H.P., Kyrpides, N., Liebl, W., Liesegang, H., Meincke, L., Pati, A., Petersen, J., Piekarski, T., Pommerenke, C., Pradella, S., Pukall, R., Rabus, R., Stackebrandt, E., Thole, S., Thompson, L., Tielen, P., Tomasch, J., von Jan, M., Wanphrut, N., Wichels, A., Zech, H., and Simon, M. (2010). The complete genome sequence of the algal symbiont Dinoroseobacter shibae: a hitchhiker's guide to life in the sea. ISME J. 4:61-77.


Katzke, N., Arvani, S., Bergmann, R., Circolone, F., Markert, A., Svensson, V., Jaeger, K.-E., Heck, A., and Drepper, T. (2010). A novel T7 RNA polymerase dependent expression system for high-level protein production in the phototrophic bacterium Rhodobacter capsulatus. Protein Expr. Purif. 69:137-146.


Piekarski T, Buchholz I, Drepper T, Schobert M, Wagner-Doebler I, Tielen P, Jahn D. (2009). Genetic tools for the investigation of Roseobacter clade bacteria. BMC Microbiol. 9:265.

Jentzsch, K., Wirtz, A., Circolone, F., Drepper, T., Losi, A., Gärtner, W., Jaeger, K.-E., Krauss, U. (2009). Mutual Exchange of Kinetic Properties by Extended Mutagenesis in Two Short LOV Domain Proteins from Pseudomonas putida. Biochemistry. 48:10321-10333.


Tremblay, P.-L., Drepper, T., Masepohl, B., and Hallenbeck, P.C. (2008). Membrane Sequestration of PII Proteins and Nitrogenase Regulation in Rhodobacter capsulatus. In: F.D. Dakora et al. (eds.). Biological Nitrogen Fixation: Towards Poverty Alleviation through Sustainable Agriculture. Springer Science and Business Media B.V.


Meier, R., Drepper, T., Svensson, V., Jaeger, K.-E., and Baumann, U. (2007). A calcium-gated lid and a large β-roll sandwich are revealed by the crystal structure of extracellular lipase from Serratia marcescens. J. Biol. Chem. 282: 31477-31483


Tremblay, P.-L., Drepper, T., Masepohl, B., and Hallenbeck, P.C. (2007). Membrane Sequestration of PII Proteins and Nitrogenase Regulation in the photosynthetic bacterium Rhodobacter capsulatus. J. Bacteriol. 189: 5850-5859


Drepper, T., Circolone, F., Heck, A., Krauss, U., and Jaeger, K.-E. (2007). Bakterien sehen Blau – Neues über LOV-Photorezeptoren. BIOspektrum 04: 361-363


Drepper, T., Eggert, T., Circolone, F., Heck, A., Krauß, U., Guterl, J.-K., Wendorff, M., Losi, A, Gärtner, W., and Jaeger, K.-E. (2007) Reporter proteins for in vivo fluorescence without oxygen. Nat. Biotechnol. 25: 443-445:


Drepper, T., Eggert, T., Hummel, W., Leggewie, C., Pohl, M., Rosenau, F., Wilhelm, S., and Jaeger, K.-E. (2006). Novel biocatalysts for white biotechnology. Biotechnol. J. 1: 777-786


Drepper, T., Wiethaus, J., Giaourakis, D., Groß, S., Schubert, B., Vogt, M., Wiencek, Y., McEwan, A. G., and Masepohl, B. (2006). Cross-talk towards the response regulator NtrC controlling nitrogen metabolism in Rhodobacter capsulatus. FEMS Microbiol. Lett. 258: 250-256


Drepper, T., Arvani, S., Rosenau, F., Wilhelm, S., and Jaeger, K.-E. (2005). High-level transcription of large gene regions: a novel T7 RNA-polymerase-based system for expression of functional hydrogenases in the phototrophic bacterium Rhodobacter capsulatus. Biochem. Soc. Trans. 33: 56-58


Sicking, C., Brusch, M., Lindackers, A., Riedel, K.-U., Schubert, B., Isakovic, N., Krall, C., Klipp, W., Drepper, T., Schneider, K., and Masepohl, B. (2005). Identification of two new genes involved in diazotrophic growth via the alternative Fe-only nitrogenase in the phototrophic purple bacterium Rhodobacter capsulatus. J. Bacteriol. 187: 92-98


Masepohl, B., Aktas, M., Brusch, M., Drepper, T., Schubert, B., Sicking, C., Vermöhlen, S., Wiethaus, J. & Schneider, K. 2005: Regulation of nitrogen fixation in Rhodobacter capsulatus, 95. In Current Plant Science and Biotechnology in Agriculture, Biological Nitrogen Fixation, Sustainable Agriculture and the Environment (eds Wang, Y. P., Lin, M., Tian, Z. X., Elmerich, C., & Newton, W. E.). Dordrecht, NL: Springer.


Masepohl, B., Drepper, T., and Klipp, W. (2004). Nitrogen fixation in the phototrophic purple bacterium Rhodobacter capsulatus. In: Genetics and regulation of nitrogen fixation in free-living bacteria, vol. II (Klipp, W., Masepohl, B., Gallon, J. R. and Newton, W. E., eds.), pp. 141-173, Kluwer Academic Publischer


Pawlowski, A., Riedel, K.-U., Klipp, W., Dreiskemper, P., Groß, S., Bierhoff, H., Drepper, T., and Masepohl, B. (2003). Yeast two-hybrid studies on interaction of proteins involved in regulation of nitrogen fixation in the phototrophic bacterium Rhodobacter capsulatus. J. Bacteriol. 185: 5240-5247.


Drepper, T., Gross, S.,Yakunin, A. F.,Hallenbeck, P. C., Masepohl, B., and Klipp, W. (2003). Role of GlnB and GlnK in ammonium control of both nitrogenase systems in the phototrophic bacterium Rhodobacter capsulatus. Microbiology 149: 2203-2212


Drepper, T., Masepohl, B., Paschen, A., Groß, S., Pawlowski, A., Raabe, K., Riedel, K.-U., and Klipp, W. (2003). Regulation of synthesis and activity of molybdenum nitrogenase and the alternative nitrogenase in Rhodobacter capsulatus. In: Dürre P., Friedrich B. (ed). Regulatory Networks in Prokaryotes. Horizon Scientific Press pp. 73-77


Masepohl, B., Schneider, K., Drepper, T., Müller, A., and Klipp, W. (2002). Alternative nitrogenases. In: Leigh J. (ed). Nitrogen Fixation at the Millenium, Elsevier Science B.V. pp. 191-222


Raabe, K., Drepper, T., Riedel, K.-U., Masepohl, B., and Klipp, W. (2002). The H-NS-like protein HvrA modulates expression of nitrogen fixation genes in the phototrophic purple bacterium Rhodobacter capsulatus by binding to selected promoters. FEMS Microbiol. Lett. 216:151-158


Drepper, T., Raabe, K., Giaourakis, D., Gendrullis, M., Masepohl, B., and Klipp, W. (2002). The Hfq-like protein NrfA of the phototrophic purple bacterium Rhodobacter capsulatus controls nitrogen fixation via regulation of nifA and anfA expression. FEMS Microbiol. Lett. 215: 221-227.


Masepohl, B., Drepper, T., Paschen, A., Groß, S., Pawlowski, A., Raabe, K., Riedel, K.-U., and Klipp, W. (2002). Regulation of nitrogen fixation in the phototrophic purple bacterium Rhodobacter capsulatus. J. Mol. Microbiol. Biotechnol. 4:243-248.


Paschen, A., Drepper, T., Masepohl, B., and Klipp, W. (2001). Rhodobacter capsulatus nifA mutants mediating nif gene expression in the presence of ammonium. FEMS Microbiol. Lett. 200: 207-213