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ORCID:      0000-0002-0096-8084

Publications March 2024


Bitzenhofer N.L., Höfel C., Thies S., Weiler A.J., Eberlein C., Heipieper H.J., Batra-Safferling R., Sundermeyer P., Heidler T., Sachse C., Busche T., Kalinowski J., Belthle T., Drepper T., Jaeger K.-E. and Loeschcke A.* (2024): Exploring engineered vesiculation by Pseudomonas putida KT2440 for natural product biosynthesis. Microbial Biotechnol 17: e14312, doi: 10.1111/1751-7915.14312.



Lenz P., Bakkes P.J., Müller C. Malek M., Freudl R., Oldiges M., Drepper T., Jaeger K.-E.* and Knapp A.* (2023): Analysis of protein secretion in Bacillus subtilis by combining a secretion stress biosensor strain with an in vivo split GFP assay. Microb Cell Fact 22: 203, doi: 10.1186/s12934-023-02199-8.


Kasahara K., Leygeber, M., Seiffarth  J., Ruzaeva  K., Drepper  T., Nöh  K., Kohlheyer  D. (2023). Enabling oxygen-controlled microfluidic cultures for spatiotemporal microbial single-cell analysis. Front Microbiol 14:1198170. doi: 10.3389/fmicb.2023.1198170.


Kossmann D.F. …, Huang M. …, Weihmann R. …, Xiao X., Gätgens F., Weber T.M., Brass H.U.C., Bitzenhofer N.L., Ibrahim S., Bangert K., Rehling L., Mueller C., Tiso T., Blank L.M., Drepper T., Jaeger K.-E., Grundler F.M.W., Pietruszka J.*, Schleker A.S.S.* and Loeschcke A.* (2023): Production of tailored hydroxylated prodiginine showing combinatorial activity with rhamnolipids against plant-parasitic nematodes. Front Microbiol 14: 1151882, doi: 10.3389/fmicb.2023.1151882.


Weihmann R., Kubicki S., Bitzenhofer N.L.; Domröse A.; Bator I., Kirschen L.-M., Kofler F., Funk A., Tiso T., Blank L., Jaeger K.-E., Drepper T., Thies S.*, Loeschcke A.* (2022). The modular pYT vector series employed for chromosomal gene integration and expression to produce carbazoles and glycolipids in P. putida. FEMS Microbes, 2023, 4, 1–17. DOI: 10.1093/femsmc/xtac030


Bitzenhofer N.L., Hilgers F., Bosio G.N., Torra J., Casini G., Beinlich F.R.M., Knieps-Grünhagen E., Gordeliy V., Jaeger K.-E., Nonell S.*, Krauss U.*, Gensch T.*, Drepper T.* (2023): Development and characterization of flavin-binding fluorescent proteins – Part II – advanced characterization. Methods Mol. Biol. 2564:143-183. doi: 10.1007/978-1-0716-2667-2_7



Hilgers F., Hogenkamp F., Klaus O., Kruse L., Loeschcke A, Bier C., Binder D., Jaeger K.-E., Pietruszka J.*, Drepper T.* (2022): Light-mediated control of gene expression in the anoxygenic phototrophic bacterium Rhodobacter capsulatus using photocaged inducers. Front. Bioeng. Biotechnol. 10:902059. Doi: 10.3389/fbioe.2022.902059


Klaus O., Hilgers F., Nakielski A., Hasenklever D., Jaeger K.-E., Axmann I.*, Drepper T.* (2022): Engineering phototrophic bacteria for the production of terpenoids. Curr. Opin. Biotechnol. 77:102764. doi: 10.1016/j.copbio.2022.102764


Gerlach T., Schain J., Nsiah B. A., van Schie M. M. C. H., Hilgers F., Bitzenhofer N. L., Drepper T., Rother D.* (2022): Photo-regulation of Enzyme Activity: The Inactivation of a Carboligase with Genetically Encoded Photosensitizer Fusion Tags. Front. Catal. 2:835919. doi: 10.3389/fctls.2022.835919


Loeschcke A., Hage-Hülsmann J., Troost K., Wewer V., Jaeger K.-E., Drepper T. (2022): Heterologous production of plant terpenes in the photosynthetic bacterium Rhodobacter capsulatus. In Plant synthetic biology: Methods and protocols. Edited by M. Zurbriggen. Methods Mol Biol, 2379: 125-154. doi: 10.1007/978-1-0716-1791-5_8.


Hogenkamp F., Hilgers F., Bitzenhofer N. L., Ophoven V., Haase M.,Bier C, Binder D., Jaeger K.-E., Drepper T.*, Pietruszka J.* (2022): Optochemical control of bacterial gene expression – novel photocaged compounds for different promoter systems. Chembiochem. 23:e202100467. doi: 10.1002/cbic.202100467.



Lenz P., Hilgers F., Burmeister A., Zimmermann L., Volkenborn K., Grünberger A., Kohlheyer D., Drepper T., Jaeger K.-E., Knapp A. (2021): The iSplit GFP assay detects intracellular recombinant proteins in Bacillus subtilis. Microb Cell Fact 20:174, doi: 10.1186/s12934-021-01663-7.


Ölçücü G., Klaus O., Jaeger K.-E., Drepper T.*, Krauss U.* (2021): Emerging Solutions for in Vivo Biocatalyst Immobilization: Tailor-Made Catalysts for Industrial Biocatalysis. ACS Sustainable Chem Eng 9: 8919−8945, doi: 10.1021/acssuschemeng.1c02045


Hage-Hülsmann J., Klaus O., Linke K., Troost K., Gora L., Hilgers F., Wirtz A., Santiago-Schübel B., Loeschcke A.*, Jaeger K.-E., Drepper T.* (2021): Production of C20, C30 and C40 terpenes in the engineered phototrophic bacterium Rhodobacter capsulatus. J Biotechnol 338: 20-30, doi: 10.1016/j.jbiotec.2021.07.002.


Hogenkamp F., Hilgers F., Knapp A., Klaus O., Bier C., Binder, D., Jaeger K.-E., Drepper T.*, Pietruszka J.* (2021): Effect of Photocaged Isopropyl β-d-1-thiogalactopyranoside Solubility on the Light Responsiveness of LacI-controlled Expression Systems in Different Bacteria. Chembiochem 22: 539-547. doi: 10.1002/cbic.202000377.


Chernomor O., Peters L., Schneidewind J., Loeschcke, A., Knieps-Grünhagen E., Schmitz F., von Lieres E., Kutta R.J., Svensson V., Jaeger K.-E., Drepper T., von Haeseler A., Krauss U*. (2021): Complex Evolution of Light-Dependent Protochlorophyllide Oxidoreductases in Aerobic Anoxygenic Phototrophs: Origin, Phylogeny, and Function. Mol Biol Evol 38: 819-837. doi: 10.1093/molbev/msaa234.


Hilgers F., Habash S.S., Loeschcke A., Ackermann Y.S., Neumann S., Heck A., Klaus O., Hage-Hülsmann J., Grundler F.M.W., Jaeger K.-E., Schleker A.S.S.*, Drepper T.* (2021): Heterologous Production of β-Caryophyllene and Evaluation of Its Activity against Plant Pathogenic Fungi. Microorganisms 9:168, doi: 10.3390/microorganisms9010168.



Habash S.S., Könen P.P., Loeschcke A., Wüst M., Jaeger K.-E., Drepper T., Grundler FWM, Schleker A.S.S. (2020): The Plant Sesquiterpene Nootkatone Efficiently Reduces Heterodera schachtii Parasitism by Activating Plant Defense. Int J Mol Sci 21: 9627, doi: 10.3390/ijms21249627.


Raber H.F., Heerde T, Nour El Din S., Flaig C., Hilgers F., Bitzenhofer N., Jaeger K.-E., Drepper T., Gottschalk K.-E., Bodenberger N.E., Weil T., Kubiczek D.H., Rosenau F. (2020): Azulitox-A Pseudomonas aeruginosa P28-Derived Cancer-Cell-Specific Protein Photosensitizer. Biomacromolecules 21: 5067-5076, doi: 10.1021/acs.biomac.0c01216.


Weihmann R., Domröse A., Drepper T., Jaeger K.-E., Loeschcke A.* (2020): Protocols for yTREX/Tn5-based gene cluster expression in Pseudomonas putida. Microb Biotechnol 13: 250-262, doi: 10.1111/1751-7915.13402.



Hage-Hülsmann J., Metzger S., Wewer V., Buechel F., Troost K., Thies S., Loeschcke A.*, Jaeger K.-E., Drepper T.* (2019): Biosynthesis of cycloartenol by expression of plant and bacterial oxidosqualene cyclases in engineered Rhodobacter capsulatus. J Biotechnol, 306S:100014, doi: 10.1016/j.btecx.2020.100014.


Hilgers F., Bitzenhofer N.L., Ackermann Y., Burmeister A., Grünberger A., Jaeger K.-E., Drepper T.* (2019): Genetically encoded photosensitizers as light-triggered antimicrobial agents. Int J Mol Sci 20: 4608, doi: 10.3390/ijms20184608.


Troost K., Loeschcke A.*, Hilgers F., Özgür A., Weber T.M., Santiago-Schübel B., Svensson V., Hage-Hülsmann J., Habash S., Grundler F.M.W., Schleker S., Jaeger K.-E., Drepper T.* (2019): Engineered Rhodobacter capsulatus as a phototrophic platform organism for the synthesis of plant sesquiterpenoids. Front Microbiol 10:1998, doi: 10.3389/fmicb.2019.01998.)


Otto M., Wynands B., Drepper T., Jaeger K.-E., Thies S., Loeschcke A., Blank L.M., Wierckx N.*  (2019): Targeting 16S rDNA for stable recombinant gene expression in Pseudomonas. ACS Synth Biol 8: 1901-1912, doi: 10.1021/acssynbio.9b00195.


Domröse A., Hage-Hülsmann J., Thies S., Weihmann R., Kruse L., Otto M., Wierckx N., Jaeger
K.-E., Drepper T.*, Loeschcke A.* (2019): Pseudomonas putida rDNA as an exceptionally suitable site for the expression of biosynthetic genes. Sci Rep 9: 7028, doi: 10.1038/s41598-019-43405-1.


Kallscheuer N., Classen T., Drepper T., Marienhagen J.* (2019): Production of plant metabolites with applications in the food industry using engineered microorganisms. Curr Opin Biotechnol 56: 7-17, doi: 10.1016/j.copbio.2018.07.008.


Peters L., Weidenfeld I., Klemm U., Loeschcke A., Weihmann R., Jaeger K.-E., Drepper T.*, Ntziachristos V., Stiel A.C.* (2019): Phototrophic purple bacteria as optoacoustic in vivo reporters of macrophage activity. Nat Comm 10: 1191, doi: 10.1038/s41467-019-09081-5.


Burmeister A., Hilgers F., Rudat A., Westerwalbesloh C., Kerkhoff Y., Drepper T., Kohlheyer D., Noack S., Grünberger A.* (2019): A microfluidic co-cultivation platform to investigate interactions in microbial communities on single-cell level. Lab Chip 19: 98, doi: 10.1039/c8lc00977e.



Endres S., Wingen M., Torra J., Ruiz-González R., Polen T., Bosio G., Bitzenhofer N.L., Hilgers F., Gensch T., Nonell S.*, Jaeger K.-E., Drepper T.* (2018): An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria. Sci Rep 8: 15021, doi: 10.1038/s41598-018-33291-4.


Hage-Hülsmann J., Grünberger A., Thies S., Santiago-Schübel B., Klein A.S., Pietruszka J., Binder D., Hilgers F., Domröse A., Drepper T., Kohlheyer D., Jaeger K.-E., Loeschcke A.* (2018): Natural biocide cocktails: combinatorial antibiotic effects of prodigiosin and biosurfactants. PLoS ONE 13: e0200940, doi: 10.1371/journal.pone.0200940.


Fettweiss T., Röllen K., Granzin J., Reiners O., Endres S., Drepper T., Willbold D., Jaeger K.-E., Batra-Safferling R.*, Krauss U.* (2018): Mechanistic basis of the fast dark recovery of the short LOV protein DsLOV from Dinoroseobacter shibae. Biochemistry 57: 4833-4847, doi: 10.1021/acs.biochem.8b00645.


Klein A.S., Brass H.U.C., Klebl D.P., Classen T., Loeschcke A., Drepper T., Sievers S., Jaeger K.-E., Pietruszka J.* (2018): Preparation of cyclic prodiginines by mutasynthesis in Pseudomonas putida KT2440. Chembiochem 19: 1545-1552, doi: 10.1002/cbic.201800154.

4Cover feature Chembiochem 14/2018, doi: 10.1002/cbic.201800328.



Loeschcke A., Dienst D., Wewer V., Hage-Hülsmann J., Dietsch M., Kranz-Finger S., Hüren V., Metzger S., Urlacher V.B., Gigolashvili T., Kopriva S., Axmann I.M.*, Drepper T.*, Jaeger K.-E. (2017): The photosynthetic bacteria Rhodobacter capsulatus and Synechocystis sp. PCC 6803 as new hosts for cyclic plant triterpene biosynthesis. PLoS ONE 12: e0189816, doi: 10.1371/journal.pone.0189816.)


Domröse A., Weihmann R., Thies S., Jaeger K.-E., Drepper T., Loeschcke A.* (2017): Rapid generation of recombinant Pseudomonas putida secondary metabolite producers using yTREX. Synth Syst Biotechno 2: 310-319, doi: 10.1016/j.synbio.2017.11.001.


Binder D.*, Drepper T., Jaeger K.-E., Delvigne F., Wiechert W., Kohlheyer D., Grünberger A.* (2017): Homogenizing bacterial cell factories: analysis and engineering of phenotypic heterogeneity. Metab Eng 42: 145-156, doi: 10.1016/j.ymben.2017.06.009.


Klein A.S., Domröse S., Bongen P., Brass H.U.C., Classen T.,  Loeschcke A., Drepper T., Laraia L., Sievers S., Jaeger K.-E., Pietruszka J.* (2017): New Prodigiosin derivatives obtained by mutasynthesis in Pseudomonas putida. ACS Synth Biol 6: 1757-1765, doi: 10.1021/acssynbio.7b00099.


Rupprecht C., Wingen M., Potzkei J., Gensch T. Jaeger K.-E., Drepper T.* (2017): A novel FbFP-based biosensor toolbox for sensitive in vivo determination of intracellular pH. J Biotechnol 258: 25-32, doi: 10.1016/j.jbiotec.2017.05.006.


Heck A. & Drepper T. (2017): Engineering Photosynthetic α-Proteobacteria for the Production of Recombinant Proteins and Terpenoids. In Modern topics in the phototrophic prokaryotes – Environmental and applied aspects. Edited by P. C. Hallenbeck, 395-425. Cham/CH, Springer International Publishing, doi: 10.1007/978-3-319-46261-5_12.


Wingen M., Jaeger K.-E., Gensch T.*, Drepper T.* (2017): Novel thermostable flavin-binding fluorescent proteins from thermophilic organisms. Photochem Photobiol 93: 849-856, doi: 10.1111/php.12740.


Katzke N., Knapp A., Loeschcke A., Drepper T., Jaeger K.-E. (2017): Novel Tools for the Functional Expression of Metagenomic DNA. In Metagenomics: Methods and protocols. 2nd edn, edited by W. R. Streit & R. Daniel. Methods Mol Biol 1539: 159-196. New York/USA, Humana Press, doi: 10.1007/978-1-4939-6691-2_10.


Bier C., Binder D., Drobietz D., Loeschcke A., Drepper T., Jaeger K.-E., Pietruszka J.* (2017): Photo-caged carbohydrates – versatile tools for controlling gene expression by light. Synthesis 49: 42-52, doi: 10.1055/s-0035-1562617.



Binder D., Frohwitter J., Mahr R., Bier C., Grünberger A., Loeschcke A., Peters-Wendisch P., Kohlheyer D., Pietruszka J., Frunzke J., Jaeger K.-E., Wendisch V.F.*, Drepper T.* (2016): Light-controlled cell factories - employing photocaged IPTG for light-mediated optimization of lac-based gene expression and (+)-valencene biosynthesis in Corynebacterium glutamicum. Appl Environ Microbiol 82: 6141-6149, doi: 10.1128/AEM.01457-16.


Binder D., Probst C., Grünberger A., Hilgers F., Loeschcke A., Jaeger K.-E., Kohlheyer D., Drepper T.* (2016): Comparative single-cell analysis of different E. coli expression systems during microfluidic cultivation. PLoS ONE 11: e0160711, doi: 10.1371/journal.pone.0160711.


Shah R., Pathak G.P., Drepper T., Gärtner W.* (2016): Selective photoreceptor gene knock-out reveals a regulatory role for the growth behavior of Pseudomonas syringae. Photochem Photobiol 92: 571-578, doi: 10.1111/php.12610.


Wandrey G., Bier C., Binder D., Hoffmann K., Jaeger K.-E., Pietruszka J., Drepper T., Büchs J.* (2016): Light-induced gene expression with photocaged IPTG for induction profiling in a high-throughput screening system. Microb Cell Fact 15: 63, doi: 10.1186/s12934-016-0461-3.


Davari M.D.*, Kopka B., Wingen M., Bocola M., Drepper T., Jaeger, K.-E., Schwaneberg U., Krauss U.* (2016): Photophysics of the LOV-based fluorescent protein variant iLOVQ489K determined by simulation and experiment. J Phys Chem B 120: 3344-3352. doi: 10.1021/acs.jpcb.6b01512.


Binder D., Bier C., Grünberger A., Drobietz D., Hage-Hülsmann J., Wandrey G., Büchs J., Kohlheyer D., Loeschcke A., Wiechert W., Jaeger K.-E., Pietruszka J., Drepper T.* (2016): Photocaged Arabinose – A Novel Optogenetic Switch for Rapid and Gradual Control of Microbial Gene Expression. Chembiochem 17: 296-299, doi: 10.1002/cbic.201500609.

4Editor’s choice: Spotlights from ChemPubSoc Europe

4Angewandte Chemie – Top-Beiträge aus unseren Schwesterzeitschriften



Domröse A., Klein A., Hage-Hülsmann J., Thies S., Svensson V., Classen T., Pietruszka J., Jaeger K.-E., Drepper T.*, Loeschcke A.* (2015): Efficient recombinant production of prodigiosin in Pseudomonas putida. Front Microbiol 6: 972, doi: 10.3389/fmicb.2015.00972.


Torra J., Burgos-Caminal A., Endres S., Wingen M., Drepper T., Gensch T., Ruiz-González R., Nonell S. (2015): Singlet oxygen and flavin-binding fluorescent proteins: a deadly tandem in LOV. FEBS J 282: 243, doi 10.1111/febs.13339.


Endres S., Granzin J., Circolone F., Stadler A., Krauss U., Drepper T., Svensson V., Knieps-Grünhagen E., Wirtz A., Cousin A., Tielen P., Willbold D., Jaeger K.-E.*, Batra-Safferling R.* (2015): Structure and function of a short LOV protein from the marine phototrophic bacterium Dinoroseobacter shibae. BMC Microbiol 15: 30, doi: 10.1186/s12866-015-0365-0.


Torra J., Burgos-Caminal A., Endres S., Wingen M., Drepper T., Gensch T., Ruiz-González R., Nonell S.* (2015): Singlet oxygen photosensitisation by the fluorescent protein Pp2FbFP L30M, a novel derivative of Pseudomonas putida flavin-binding Pp2FbFP. Photochem Photobiol Sci 14: 280-287, doi 10.1039/c4pp00338a.



Drepper T.*, Funken H., Heck A., Kovacic F., Krauss U., Pietruszka J. (2014): Editorial - Special Issue dedicated to Karl-Erich Jaeger on the occasion of his 60th birthday. J Biotechnol 191: 1-2, doi: doi:10.1016/j.jbiotec.2014.10.011.


Liebl W.*, Angelov A., Juergensen J., Chow J., Loeschcke A., Drepper T., Classen T., Pietruszka J., Ehrenreich A., Streit W.R., Jaeger K.-E. (2014): Alternative hosts for functional (meta)genome analysis. Appl Microbiol Biotechnol 98: 8099-8109, doi: 10.1007/s00253-014-5961-7.


Binder D., Grünberger A., Loeschcke A. Probst C., Pietruszka J., Wiechert W., Kohlheyer D., Jaeger K.-E.*, Drepper T.* (2014): Light-responsive control of bacterial gene expression: Precise triggering of the lac promoter activity using photocaged IPTG. Integr Biol 6: 755-765, doi: 10.1039/c4ib00027g.


Kaschner M., Loeschcke A., Krause J., Minhc B.Q., Heck A., Svensson V., Wirtz A., von Haeseler A., Jaeger K.-E., Drepper T.*, Krauss, U.* (2014): Discovery of the first light-dependent protochlorophyllide oxidoreductase in anoxygenic phototrophic bacteria. Mol Microbiol 93: 1066-1078, doi: 10.1111/mmi.12719.


Wingen M., Potzkei J., Endres S., Casini G., Rupprecht C., Fahlke C., Krauss U., Jaeger K.-E., Drepper T.*, Gensch T.* (2014): The photophysics of LOV-based fluorescent proteins – new tools for cell biology. Photochem Photobiol Sci 13: 875-883, doi: 10.1039/c3pp50414j.



Drepper T.*, Gensch T.*, Pohl, M. (2013): Advanced in vivo applications of blue light photoreceptors as alternative fluorescent proteins. Photochem Photobiol Sci 12: 1125-1134, doi: 10.1039/c3pp50040c.


Loeschcke A., Markert A., Wilhelm S., Wirtz A., Rosenau F., Jaeger K.-E.*, Drepper T.* (2013): TREX – A Universal Tool for the Transfer and Expression of Biosynthetic Pathways in Bacteria. ACS Synth Biol 2, 22–33, doi: 10.1021/sb3000657.



Katzke N., Jaeger K.-E., Drepper, T. (2012): High-level gene expression in the photosynthetic bacterium Rhodobacter capsulatus. In Recombinant gene expression. 3rd edn, edited by A. Lorence. Methods Mol Biol 824: 251-269. New York/USA, Humana Press, doi: 10.1007/978-1-61779-433-9_13.


Drepper T.*, Heck A., Jaeger K.-E.* (2012): Neue Wege für eine nachhaltige Biotechnologie - Heterologe Genexpression in photosynthetischen Bakterien. systembiologie.de 5: 46-50.


Walter J.*, Hausmann S., Drepper T., Puls M., Eggert T., Dihné M. (2012): Flavin mononucleotide-based fluorescent proteins function in mammalian cells without oxygen requirement. PLoS One 7: e43921, doi: 10.1371/journal.pone.0043921.


Arvani S., Markert A., Loeschcke A., Jaeger K.-E., Drepper T.* (2012): A T7 RNA polymerase-based toolkit for the concerted expression of clustered genes. J Biotechnol 159: 162-171, doi: 10.1016/j.jbiotec.2012.01.004.


Potzkei J., Kunze M., Drepper T.*, Gensch T., Jaeger K.-E.*, Büchs J. (2012): Real-time determination of intracellular oxygen using a genetically encoded FRET-based biosensor. BMC Biology 10: 28, doi: 10.1186/1741-7007-10-28. (


Circolone F., Granzin J., Jentzsch K., Drepper T., Jaeger K.-E., Willbold D., Krauss U.*, Batra-Safferling R.* (2012): Structural basis for the slow dark recovery of a full-length LOV protein from Pseudomonas putida. J Mol Biol 417: 362–374, doi: 10.1016/j.jmb.2012.01.056.



Drepper T.*, Krauss U., Meyer zu Berstenhorst S., Pietruszka J., Jaeger K.-E. (2011): Lights on and action! - How to control microbial gene expression by light. Appl Microbiol Biotechnol 90: 23-40, doi: 10.1007/s00253-011-3141-6.


Krauss U.*, Drepper T., Jaeger K.-E. (2011): Enlightened enzymes: Strategies to create novel photoresponsive proteins. Chem Eur J 17(9): 2552-2560, doi: 10.1002/chem.201002716.



Wagner-Döbler I.*, Ballhausen B., Berger M., Brinkhoff T., Buchholz I., Bunk B., Cypionka H., Daniel R., Drepper T., Gerdts G., Hahnke S., Han C., Jahn D., Kalhoefer D., Kiss H., Klenk H.-P., Kyrpides N., Liebl W., Liesegang H., Meincke L., Pati A., Petersen J., Piekarski T., Pommerenke C., Pradella S., Pukall R., Rabus R., Stackebrandt E., Thole S., Thompson L., Tielen P., Tomasch J., von Jan M., Wanphrut N., Wichels A., Zech H., Simon, M. (2010): The complete genome sequence of the algal symbiont Dinoroseobacter shibae: a hitchhiker's guide to life in the sea. ISME J 4: 61-77 doi: 10.1038/ismej.2009.94.


Troeschel S.C., Drepper T., Leggewie C., Streit W., Jaeger K.-E. (2010): Novel tools for the functional expression of metagenomic DNA. In Metagenomics – Methods and Protocols. Edited by W. R. Streit and R. Daniel. Methods Mol Biol 668: 117-139. New York/USA, Humana Press, doi: 10.1007/978-1-60761-823-2_8.


Drepper T.*, Huber R., Heck A., Circolone F., Hillmer A.-K., Büchs J., Jaeger K.-E.* (2010): Flavin mononucleotide-based fluorescent reporter proteins outperform green fluorescent protein-like proteins as quantitative in vivo real-time reporters. Appl Environ Microbiol 76: 5990–5994, doi:  10.1128/AEM.00701-10.

4Cover illustration of Appl Envirnom Microbiol 76.


Katzke N., Arvani S., Bergmann R., Circolone F., Markert A., Svensson V., Jaeger K.-E., Heck A., Drepper, T.* (2010): A novel T7 RNA polymerase dependent expression system for high-level protein production in the phototrophic bacterium Rhodobacter capsulatus. Protein Expr Purif 69: 137-146, doi: 10.1016/j.pep.2009.08.008.



Piekarski T., Buchholz I., Drepper T., Schobert M., Wagner-Doebler I., Tielen P.*, Jahn D. (2009): Genetic tools for the investigation of Roseobacter clade bacteria. BMC Microbiol 9: 265 doi: 10.1186/1471-2180-9-265.


Jentzsch K., Wirtz A., Circolone F., Drepper T., Losi, A, Gärtner W., Jaeger K.-E., Krauss U.* (2009): Mutual Exchange of Kinetic Properties by Extended Mutagenesis in Two Short LOV Domain Proteins from Pseudomonas putida. Biochemistry 48: 10321-10333, doi: 10.1021/bi901115z.



Meier R., Drepper T., Svensson V., Jaeger K.-E., Baumann, U.* (2007): A calcium-gated lid and a large β-roll sandwich are revealed by the crystal structure of extracellular lipase from Serratia marcescens. J Biol Chem 282: 31477-31483, doi: 10.1074/jbc.M704942200.


Tremblay P.-L., Drepper T., Masepohl B., Hallenbeck P.C.* (2007): Membrane Sequestration of PII Proteins and Nitrogenase Regulation in the photosynthetic bacterium Rhodobacter capsulatus. J Bacteriol 189: 5850-5859, doi: 10.1128/JB.00680-07.


Drepper T.*, Eggert T., Circolone F., Heck A., Krauß U., Guterl J.-K., Wendorff M., Losi A, Gärtner W., Jaeger, K.-E.* (2007): Reporter proteins for in vivo fluorescence without oxygen. Nat Biotechnol 25: 443-445, doi: 10.1038/nbt1293.

4press release in transcript (4), 2007

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4press release in FZ Jülich Online, 12.03.2007

4recommended in Nature Methods 4(5), 2007, “news in brief”

4recommended by Faculty of 1000, 27.04.2007



Drepper T., Eggert T., Hummel W., Leggewie C., Pohl M., Rosenau F., Wilhelm S., Jaeger K.-E.* (2006): Novel biocatalysts for white biotechnology. Biotechnol J 1: 777-786, doi: 10.1002/biot.200600059.


Drepper T., Wiethaus J., Giaourakis D., Groß S., Schubert B., Vogt M., Wiencek Y., McEwan A.G., Masepohl, B.* (2006): Cross-talk towards the response regulator NtrC controlling nitrogen metabolism in Rhodobacter capsulatus. FEMS Microbiol Lett 258: 250-256, doi: 10.1111/j.1574-6968.2006.00228.x.



Drepper T.*, Arvani S., Rosenau F., Wilhelm S., Jaeger K.-E. (2005): High-level transcription of large gene regions: a novel T7 RNA-polymerase-based system for expression of functional hydrogenases in the phototrophic bacterium Rhodobacter capsulatus. Biochem Soc Trans 33: 56-58, doi: 10.1042/BST0330056.


Sicking C., Brusch M., Lindackers A., Riedel K.-U., Schubert B., Isakovic N., Krall C., Klipp W., Drepper T., Schneider K., Masepohl B.* (2005): Identification of two new genes involved in diazotrophic growth via thealternative Fe-only nitrogenase in the phototrophic purple bacterium Rhodobacter capsulatus. J Bacteriol 187: 92-98, doi: 10.1128/JB.187.1.92-98.2005.



Masepohl B., Drepper T., Klipp, W. (2004): Nitrogen fixation in the phototrophic purple bacterium Rhodobacter capsulatus. In Genetics and Regulation of Nitrogen Fixation in Free-Living Bacteria. Edited by W. Klipp, B. Masepohl, J. R. Gallon, W. E. Newton. Nitrogen Fixation: Origins, Applications, and Research Progress 2: 141-173. Dordrecht/NL, Springer Netherlands, doi: 10.1007/1-4020-2179-8.



Drepper T.*, Gross S., Yakunin A.F., Hallenbeck P.C., Masepohl B., Klipp, W. (2003): Role of GlnB and GlnK in ammonium control of both nitrogenase systems in the phototrophic bacterium Rhodobacter capsulatus. Microbiology 149: 2203-2212, doi: 10.1099/mic.0.26235-0.


Drepper T., Masepohl B., Paschen A., Groß S., Pawlowski A., Raabe K., Riedel K.-U., Klipp, W. (2003): Regulation of synthesis and activity of molybdenum nitrogenase and the alternative nitrogenase in Rhodobacter capsulatus. In Regulatory Networks in Prokaryotes. Edited by P. Dürre and B. Friedrich. Norwich/UK, Horizon Scientific Press, 73-77.


Pawlowski A., Riedel K.-U., Klipp W., Dreiskemper P., Groß S., Bierhoff H., Drepper T., Masepohl B.* (2003): Yeast two-hybrid studies on interaction of proteins involved in regulation of nitrogen fixation in the phototrophic bacterium Rhodobacter capsulatus. J Bacteriol 185: 5240-5247, doi: 10.1128/JB.185.17.5240-5247.2003.



Raabe K., Drepper T., Riedel K.-U., Masepohl B.*, Klipp, W. (2002): The H-NS-like protein HvrA modulates expression of nitrogen fixation genes in the phototrophic purple bacterium Rhodobacter capsulatus by binding to selected promoters. FEMS Microbiol Lett 216: 151-158, doi: 10.1111/j.1574-6968.2002.tb11429.x.


Drepper T., Raabe K., Giaourakis D., Gendrullis M., Masepohl B.*, Klipp, W. (2002): The Hfq-like protein NrfA of the phototrophic purple bacterium Rhodobacter capsulatus controls nitrogen fixation via regulation of nifA and anfA expression. FEMS Microbiol Lett 215: 221-227, doi: 10.1111/j.1574-6968.2002.tb11394.x.


Masepohl B., Drepper T., Paschen A., Groß S., Pawlowski A., Raabe K., Riedel K.-U., Klipp W.* (2002): Regulation of nitrogen fixation in the phototrophic purple bacterium Rhodobacter capsulatus. J Mol Microbiol Biotechnol 4: 243-248.


Masepohl B., Schneider K., Drepper T., Müller A., Klipp, W. (2002): Alternative nitrogenases. In Nitrogen Fixation at the Millennium. Edited by J. Leigh. Amsterdam/NL, Elsevier Science B.V., 191-222, doi: 10.1016/B978-0-444-50965-9.50018-5.



Paschen A., Drepper T., Masepohl B., Klipp W.* (2001): Rhodobacter capsulatus nifA mutants mediating nif gene expression in the presence of ammonium. FEMS Microbiol Lett 200: 207-213, doi: 10.1111/j.1574-6968.2001.tb10717.x.


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