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Public outreach

Publication

Marasco R, Fusi M, Coscolín C, Barozzi A, Almendral D, Bargiela R, Nutschel CG neé, Pfleger C, Dittrich J, Gohlke H, Matesanz R, Sanchez-Carrillo S, Mapelli F, Chernikova TN, Golyshin PN, Ferrer M, Daffonchio D (2023). Enzyme adaptation to habitat thermal legacy shapes the thermal plasticity of marine microbiomes. Nat Commun 141 14:1–15. doi: 10.1038/s41467-023-36610-0


Publication

Weihmann R, Kubicki S, Bitzenhofer NL, Domröse A, Bator I, Kirschen L-M, Kofler F, Funk A, Tiso T, Blank LM, Jaeger K-E, Drepper T, Thies S, Loeschcke A (2023). The modular pYT vector series employed for chromosomal gene integration and expression to produce carbazoles and glycolipids in P. putida. FEMS Microbes 4:1–17. doi: 10.1093/femsmc/xtac030


Publication

Han, Y., Kinfu, B. M., Blombach, F., Cackett, G., Zhang, H., Pérez-García, P., et al. (2022). A novel metagenome-derived viral RNA polymerase and its application in a cell-free expression system for metagenome screening. Sci. Rep. 12, 17882. doi:10.1038/s41598-022-22383-x.


Publication

El Harrar, T., Davari, M. D., Jaeger, K.-E., Schwaneberg, U., and Gohlke, H. (2022). Critical assessment of structure-based approaches to improve protein resistance in aqueous ionic liquids by enzyme-wide saturation mutagenesis. Comput. Struct. Biotechnol. J. 20, 399–409. doi:10.1016/j.csbj.2021.12.018.


Publication

Weigert, S., Perez‐Garcia, P., Gisdon, F. J., Gagsteiger, A., Schweinshaut, K., Ullmann, G. M., et al. (2022). Investigation of the halophilic PEThydrolase PET6 from Vibrio gazogenes. Protein Sci. 31. doi:10.1002/pro.4500.


Publication

Chow, J., Perez‐Garcia, P., Dierkes, R., and Streit, W. R. (2022). Microbial enzymes will offer limited solutions to the global plastic pollution crisis. Microb. Biotechnol. doi:10.1111/1751-7915.14135.


Publication

El Harrar, T., and Gohlke, H. (2022). Cumulative Millisecond-Long Sampling for a Comprehensive Energetic Evaluation of Aqueous Ionic Liquid Effects on Amino Acid Interactions. J. Chem. Inf. Model. doi:10.1021/acs.jcim.2c01123.


Publication

Dierkes, R. F., Wypych, A., Pérez-García, P., Danso, D., Chow, J., and Streit, W. R. (2022). An Ultra-Sensitive Comamonas thiooxidans Biosensor for the Rapid Detection of Enzymatic Polyethylene Terephthalate (PET) Degradation. Appl. Environ. Microbiol. doi:10.1128/aem.01603-22.


Publication

Antranikian, G. and Streit, W.R. (2022) Microorganisms harbor keys to a circular bioeconomy making them useful tools in fighting plastic pollution and rising CO2 levels. Extrem. 2022 261 26: 1–11. doi: 10.1007/S00792-022-01261-4


Publication

Zhang, H., Perez-Garcia, P., Dierkes, R.F., Applegate, V., Schumacher, J., Chibani, C.M., et al. (2022) The Bacteroidetes Aequorivita sp. and Kaistella jeonii Produce Promiscuous Esterases With PET-Hydrolyzing Activity. Front. Microbiol. 12: 3874. doi: 10.3389/fmicb.2021.803896


Publication

Buchholz, P.C.F., Feuerriegel, G., Zhang, H., Perez‐Garcia, P., Nover, L.-L., Chow, J., et al. (2022) Plastics degradation by hydrolytic enzymes: The plastics‐active enzymes database—PAZyProteins Struct. Funct. Bioinforma. https://doi.org/10.1002/prot.26325


Publication

El Harrar, T., Frieg, B., Davari, M.D., Jaeger, K.E., Schwaneberg, U., and Gohlke, H. (2021) Aqueous ionic liquids redistribute local enzyme stability via long-range perturbation pathways. Comput. Struct. Biotechnol. J. 19: 4248–4264. doi:10.1016/J.CSBJ.2021.07.001


Publication

Perez-Garcia, P., Kobus, S., Gertzen, C.G.W., Hoeppner, A., Holzscheck, N., Strunk, C.H., et al. (2021) A promiscuous ancestral enzyme´s structure unveils protein variable regions of the highly diverse metallo-β-lactamase family. Commun. Biol. 2021 41 4: 1–12. doi:10.1038/s42003-021-01671-8


Publication

Harrar, T. El, Davari, M.D., Jaeger, K.-E., Schwaneberg, U., and Gohlke, H. (2021) Critical assessment of structure-based approaches to improve protein resistance in aqueous ionic liquids by enzyme-wide saturation mutagenesis. Comput. Struct. Biotechnol. J. doi:10.1016/j.csbj.2021.12.018


Publication

Höppner A, Bollinger A, Kobus S, Thies S, Coscolín C, Ferrer M, Jaeger K-E, Smits SHJ. Crystal structures of a novel family IV esterase in free and substrate‐bound form. FEBS J; 2021;. 288: 3570–3584. doi:10.1111/febs.15680


Publication

Nutschel, C., Coscolín, C., David, B., Mulnaes, D., Ferrer, M., Jaeger, K.-E., and Gohlke, H. (2021) Promiscuous Esterases Counterintuitively Are Less Flexible than Specific Ones. J. Chem. Inf. Model. 61: 2383–2395. doi: 10.1021/acs.jcim.1c00152


Publication

Cui, H., Eltoukhy, L., Zhang, L., Markel, U., Jaeger, K.E., Davari, M.D., and Schwaneberg, U. (2021) Less Unfavorable Salt Bridges on the Enzyme Surface Result in More Organic Cosolvent Resistance. Angew. Chemie - Int. Ed. 60: 11448–11456. doi: 10.1002/anie.202101642


Blog-Entry

Molitor R

Blaue Biotechnologie – Eine Expedition zum Nordatlantischen Müllstrudel

JülichBlog Bioökonomie

https://blogs.fz-juelich.de/biooekonomie/2021/03/01/blaue-biotechnologie-eine-expedition-zum-nordatlantischen-muellstrudel/


Publikation

Perez-Garcia P, Kobus S, Gertzen CGW, Hoeppner A, Holzscheck N, Strunk CH, Huber H, Jaeger K-E, Gohlke H, Kovacic F, Smits SHJ, Streit W, Chow J. (2021). A promiscuous ancestral enzyme´s structure unveils protein variable regions of the highly diverse metallo-β-lactamase family. Commun Biol. 2021;4: 132. doi:10.1038/s42003-021-01671-8


Publication:
Höppner A, Bollinger A, Kobus S, Thies S, Coscolín C, Ferrer M, Jaeger K-E, Smits SHJ. Crystal structures of a novel family IV esterase in free and substrate‐bound form. FEBS J; 2020; febs.15680. doi:10.1111/febs.15680


Publication
Bollinger A, Molitor R, Thies S, Koch R, Coscolín C, Ferrer M, Jaeger K-E (2020) Organic-Solvent-Tolerant Carboxylic Ester Hydrolases for Organic Synthesis. Appl Environ Microbiol 86:e00106-20 . https://doi.org/10.1128/AEM.00106-20


Newspaper article
Forschung in Düsseldorf: So sollen die Meere sauber werden
https://rp-online.de/nrw/staedte/duesseldorf/duesseldorf-bakterien-sollen-das-meer-reinigen_aid-49560371


Publication
Bollinger A, Thies S, Knieps-Grünhagen E, Gertzen C, Kobus S, Höppner A, Ferrer M, Gohlke H, Smits SHJ, Jaeger K-E (2020) A Novel Polyester Hydrolase From the Marine Bacterium Pseudomonas aestusnigri – Structural and Functional Insights. Front Microbiol 11:114 . https://doi.org/10.3389/fmicb.2020.00114


Press release
Vier neue Projekte zur Bioökonomie an der HHU