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Publications (May 2023)


Kossmann DF, Huang M, Weihmann R, Xiao X, Gätgens F, Weber TM, Brass HU, Bitzenhofer NL, Schinya I, Bangert K, Rehling L, Müller C, Tiso T, Blank L, Drepper T, Jaeger KE, Grundler F, Pietruszka J, Schleker AS, Loeschcke A. Production of tailored hydroxylated prodiginine showing combinatorial activity with rhamnolipids against plant-parasitic nematodes. Front Microbiol. 2023;14: 1249. doi:10.3389/FMICB.2023.1151882

  Molina-Espeja P, Sanz-Aparicio J, Golyshin PN, Robles-Martín A, Guallar V, Beltrametti F, Müller M, Yakimov M, Modregger J, van Logchem M., Corvini P, Shahgaldian P, Degering C, Wieland S, Timm A, de Carvalho CCCR, Re I, Daniotti S, Thies S, Jaeger K-E, Chow J, Streit, WR, Lottenbach R Rösch R, Ansari N, Ferrer M, The FuturEnzyme consortium. Enzymes for consumer products to achieve climate neutrality. Enzymes for consumer products to achieve climate neutrality. Oxford Open Clim Chang. 2023;3: kgad003. doi:10.1093/oxfclm/kgad003
  Filbig M, Kubicki S, Bator I, Hausmann R, Blank LM, Henkel M, Thies S, Tiso T. Metabolic and process engineering on the edge—Rhamnolipids are a true challenge: A review. In: Soberón-Chávez G, editor. Biosurfactants Research and development. 1st ed. Elsevier Academic Press; 2023. ISBN 9780323985611 pp. 157–182.
  Weihmann R, Kubicki S, Bitzenhofer NL, Domröse A, Bator I, Kirschen L-M, Kofler F, Funk A, Tiso T, Blank LM, Jaeger K-E, Drepper T, Thies S, Loeschcke A. The modular pYT vector series employed for chromosomal gene integration and expression to produce carbazoles and glycolipids in P. putida. FEMS Microbes. 2023;4: 1–17. doi:10.1093/femsmc/xtac030
2022 Hilgers F, Hogenkamp F, Klaus O, Kruse L, Loeschcke A, Bier C Binder D, Jaeger K-E, Pietruszka J, Drepper T. Light-mediated control of gene expression in the anoxygenic phototrophic bacterium Rhodobacter capsulatus using photocaged inducers. Front Bioeng Biotechnol. Frontiers Media SA; 2022;10: 1783. doi:10.3389/FBIOE.2022.902059/BIBTEX
  Cui J, Hölzl G, Karmainski T, Tiso T, Kubicki S, Thies S, Blank L, Jaeger K-E, Dörmann P.The Glycine-Glucolipid of Alcanivorax borkumensis Is Resident to the Bacterial Cell Wall. Appl Environ Microbiol. 2022; 88.doi:10.1128/AEM.01126-22
  Roda S, Fernandez-Lopez L, Benedens M, Bollinger A, Thies S, Schumacher J, Coscolín C, Kazemi M, Santiago G, CGW Gertzen CGW, Gonzalez-Alfonso JL,  Plou FJ,  Jaeger K-E, Smits SHJ, Ferrer M,Guallar V. A plurizyme with transaminase and hydrolase activity catalyzes cascade reactions. Angew Chemie Int Ed. 2022; 61. doi:10.1002/ANIE.202207344
  Puiggené Ò, José M, Espinosa C, Schlosser D, Thies S, Jehmlich N, Kappelmeyer U, Schreiber S, Wibberg D, Kalinowski J, Harms H, Heipieper, HJ, Eberlein C. Extracellular degradation of a polyurethane oligomer involving outer membrane vesicles and further insights on the degradation of 2,4-diaminotoluene in Pseudomonas capeferrum TDA1. Sci Reports 2022 121. 2022;12: 1–12. doi:10.1038/s41598-022-06558-0
  Loeschcke A, Hage-Hülsmann J, Troost K, Wewer V, Jaeger K-E, Drepper T. Heterologous Production of Plant Terpenes in the Photosynthetic Bacterium Rhodobacter capsulatus. 2022; 125–154. doi:10.1007/978-1-0716-1791-5_8
  Johann S, Weichert FG, Schröer L, Stratemann L, Kämpfer C, Seiler T-B, Heger S, Töpel A, Sassmann T, Pich A, Jakob F, Schwaneberg U, Stoffels P, Philipp M, Terfrüchte M, Loeschcke A, Schipper K, Feldbrügge M, Ihling N, Büchs J, Bator I, Tiso T, Blank LM, Roß-Nickoll M, Hollert H. A plea for the integration of Green Toxicology in sustainable bioeconomy strategies – Biosurfactants and microgel-based pesticide release systems as examples. J Hazard Mater. 2022;426: 127800. doi:10.1016/j.jhazmat.2021.127800
2021 Beck A, with contributions from Erik Borchert, Louise Delaigue, Feifei Deng, Sonia Gueroun, Thea Hamm, Oliver Jacob, Mikael Kaandorp, Hashan Kokuhennadige, Elke Kossel, Sarah-Marie Kröger, Rebecka Molitor, André Mutzberg, Ulrike Panknin, Gabriella Pantó, Annalisa Sambolino, Rui Shen, Tristan Zimmermann. NAPTRAM - Plastiktransportmechanismen, Senken und Interaktionen mit Biota im Nordatlantik / NAPTRAM - North Atlantic plastic transport mechanisms, sinks, and interactions with biota, Cruise No. SO279, Emden (Germany) – Emden (Germany), 04.12.2020 – 05.01. 2021. doi:10.3289/CR_SO279
  Trindade M, Sithole N, Kubicki S, Thies S, Burger A. Screening Strategies for Biosurfactant Discovery. Springer, Berlin, Heidelberg; 2021. pp. 1–36. doi:10.1007/10_2021_174
  Hage-Hülsmann J, Klaus O, Linke K, Troost K, Gora L, Hilgers F, Wirtz A, Santiago-Schübel B, Loeschcke A, Jaeger K-E,  Drepper, T. Production of C20, C30 and C40 terpenes in the engineered phototrophic bacterium Rhodobacter capsulatus. J Biotechnol. Elsevier; 2021;338: 20–30. doi:10.1016/j.jbiotec.2021.07.002
  Bitzenhofer NL, Kruse L, Thies S, Wynands B, Lechtenberg T, Rönitz J, Kozaeva E, Wirth NT, Eberlein C, Jaeger K-E, Nikel PI, Heipieper HJ, Wierckx N, Loeschcke A. Towards robust Pseudomonas cell factories to harbour novel biosynthetic pathways. Essays Biochem. 2021;65: 319–336. doi:10.1042/EBC20200173

Hilgers F, Habash SS, Loeschcke A, Sebastian Ackermann Y, Neumann S, Heck A, Klaus O, Hage-Hülsmann J, Grundler FMW, Jaeger K-E, Schleker ASS, Drepper T. Heterologous production of β-caryophyllene and evaluation of its activity against plant pathogenic fungi. Microorganisms. 2021;9: 168. doi:10.3390/microorganisms9010168


Höppner A, Bollinger A, Kobus S, Thies S, Coscolín C, Ferrer M, Jaeger K-E, Smits SHJ. Crystal structures of a novel family IV esterase in free and substrate‐bound form. FEBS J; 2021;. 288: 3570–3584. doi:10.1111/febs.15680


Chernomor O, Peters L, Schneidewind J, Loeschcke A, Knieps-Grünhagen E, Schmitz F, , von Lieres E, Kutta R J, Svensson V, Jaeger K-E, Drepper T, von Haeseler A, Krauss, U. Complex Evolution of Light-Dependent Protochlorophyllide Oxidoreductases in Aerobic Anoxygenic Phototrophs: Origin, Phylogeny, and Function. Mol Biol Evol. 2021;38: 819–837. doi:10.1093/molbev/msaa234

2020 Molitor R. Blog entry on expedition SO279. In: Hotmic – Transport and Fate of Marine Microplastics Blog. 2020
  Habash SS, Könen PP, Loeschcke A, Wüst M, Jaeger K-E, Drepper T, Grundler FMW, Schleker ASS. The Plant Sesquiterpene Nootkatone Efficiently Reduces Heterodera schachtii Parasitism by Activating Plant Defense. Int J Mol Sci. Int J Mol Sci; 2020;21: 9627. doi:10.3390/ijms21249627


Tiso T, Ihling N, Kubicki S, Biselli A, Schonhoff A, Bator I, Loeschcke A, Zapp P, Jupke A, Jaeger K-E, Büchs J, Blank LM. Integration of genetic and process engineering for optimized rhamnolipid production using Pseudomonas putida. Front Bioeng Biotechnol. 2020;8: 976. doi:10.3389/fbioe.2020.00976

Kubicki S, Bator I, Jankowski S, Schipper K, Tiso T, Feldbrügge M, Blank LM, Thies S, Jaeger K-E. A straightforward assay for screening and quantification of biosurfactants in microbial culture supernatants. Front Bioeng Biotechnol. 2020;8: 958. doi:10.3389/fbioe.2020.00958

Lee J, Hilgers F, Loeschke A, Jaeger K-E, Feldbrügge M. Ustilago maydis serves as a novel production host for the synthesis of plant and fungal sesquiterpenoids. Front Microbiol. 2020;11: 1655. doi:10.3389/fmicb.2020.01655

Loeschcke A, Thies S. Engineering of natural product biosynthesis in Pseudomonas putida.
Curr Opin Biotechnol. 2020;65: 213–224. doi:10.1016/j.copbio.2020.03.007

Bollinger A, Molitor R, Thies S, Koch R, Coscolín C, Ferrer M, Jaeger K-E. Identification of organic solvent tolerant carboxylic ester hydrolases for organic synthesis. Appl Environ Microbiol. 2020; doi:10.1128/AEM.00106-20

Hage-Hülsmann J, Metzger S, Wewer V, Buechel F, Troost K, Thies S, Loeschcke A., Jaeger K-E, Drepper T. Biosynthesis of cycloartenol by expression of plant and bacterial oxidosqualene cyclases in engineered Rhodobacter capsulatus.; J Biotechnol X. 2019;4: 100014. doi:10.1016/j.btecx.2020.100014

Bollinger A, Thies S, Knieps-Grünhagen E, Gertzen C, Kobus S, Höppner A, Ferrer M, Gohlke H, Smits SH, Jaeger K-E. A novel polyester hydrolase from the marine bacterium Pseudomonas aestusnigri -structural and functional insights. Front Microbiol. 2020;11: 114. doi:10.3389/FMICB.2020.00114

Molitor R, Bollinger A, Kubicki S, Loeschcke A, Jaeger K-E, Thies S. Agar plate-based screening methods for the identification of polyester hydrolysis by Pseudomonas species. Microb Biotechnol. 2020;13: 274–284. doi:10.1111/1751-7915.13418

Weihmann R, Domröse A, Drepper T, Jaeger K-E, Loeschcke A. Protocols for yTREX/Tn5-based gene cluster expression in Pseudomonas putida. Microb Biotechnol. 2020;13: 250–262. doi:10.1111/1751-7915.13402

Bollinger A, Thies S, Katzke N, Jaeger K-E. The biotechnological potential of marine bacteria in the novel lineage of Pseudomonas pertucinogena. Microb Biotechnol. 2020;13: 19–31. doi:10.1111/1751-7915.13288


Novel carboxyesterhydrolases. EP3572523A1, 2019. Available: patents.google.com/patent/EP3572523A1

Troost K, Loeschcke A, Hilgers F, Özgür AY, Weber TM, Santiago-Schübel B, Svensson V, Hage-Hülsmann J, Habash S, Grundler F, Schleker S, Jaeger K-E, Drepper T. Engineered Rhodobacter capsulatus as a phototrophic platform organism for the synthesis of plant sesquiterpenoids. Front Microbiol. 2019;10: 1998. doi:10.3389/FMICB.2019.01998

Otto M, Wynands B, Drepper T, Jaeger K-E, Thies S, Loeschcke A, Blank LM, Wierckx N. Targeting 16S rDNA for Stable Recombinant Gene Expression in Pseudomonas. ACS Synth Biol. 2019; acssynbio.9b00195. doi:10.1021/acssynbio.9b00195

Kubicki S, Bollinger A, Katzke N, Jaeger K-E, Loeschcke A, Thies S. Marine Biosurfactants: Biosynthesis, Structural Diversity and Biotechnological Applications. Mar Drugs. 2019;17: 408. doi:10.3390/md17070408

Domröse A, Hage-Hülsmann J, Thies S, Weihmann R, Kruse L, Otto M, Wierckx N, Jaeger K‑E, Drepper T, Loeschcke A. Pseudomonas putida rDNA is a favored site for the expression of biosynthetic genes. Sci Reports 2019;9: 7028. doi:10.1038/s41598-019-43405-1

Peters L, Weidenfeld I, Klemm U, Loeschcke A, Weihmann R, Jaeger K-E, Drepper T, Ntziachristos V, Stiel A C. Phototrophic purple bacteria as optoacoustic in vivo reporters of macrophage activity. Nat Commun. 2019;10: 1191. doi:10.1038/s41467-019-09081-5







Coscolín C, Katzke N, García-Moyano A, Navarro-Fernández J., Almendral D., a Martínez-Martínez M., Bollinger A., Bargiela R., Gerler C., Chernikova T., Rojo D., Barbas C, Hai T, Golyshina O, Koch R, Yakimov M, Bjerga G, Golyshin P, Jaeger K-E, and Ferrer M, the INMARE Consortium. Bioprospecting reveals class III ω-transaminases converting bulky ketones and environmentally relevant polyamines. Appl Environ Microbiol. American Society for Microbiology; 2018; AEM.02404-18. doi:10.1128/AEM.02404-18

Coscolín C, Bargiela R, Martínez-Martínez M, Alonso S, Bollinger A, Thies S, Chernikova TN, Hai T, Golyshina OV, Jaeger K-E, Yakimov MM, Golyshin PN, Ferrer M. Hydrocarbon-Degrading Microbes as Sources of New Biocatalysts. In: McGenity TJ, editor. Taxonomy, Genomics and Ecophysiology of Hydrocarbon-Degrading Microbes. Cham: Springer International Publishing; 2018. pp. 353-373. doi: 10.1007/978-3-030-14796-9_13

Kubicki S, Weihmann R, Bator I, Domröse A, Loeschcke A, Drepper T, Tiso T, Blank L, Jaeger K-E, Thies S. Konstruktion von Pseudomonas putida -Stämmen zur heterologen Produktion von Rhamnolipiden. Chemie Ing Tech. Wiley-Blackwell; 2018;90: 1282–1282. doi:10.1002/cite.201855328

Klein AS, Brass HUC, Klebl DP, Classen T, Loeschcke A, Drepper T, Sievers S, Jaeger K-., Pietruszka J. Cover Feature: Preparation of Cyclic Prodiginines by Mutasynthesis in Pseudomonas putida KT2440 (ChemBioChem 14/2018). ChemBioChem. Wiley-Blackwell; 2018;19: 1462–1462. doi:10.1002/cbic.201800328

Hage-Hülsmann J, Grünberger A, Thies S, Santiago-Schübel B, Klein AS, Pietruszka J, Binder D, Hilgers F, Domröse A, Drepper T, Kohlheyer D, Jaeger K-E, Loeschcke A. Natural biocide cocktails: Combinatorial antibiotic effects of prodigiosin and biosurfactants. PLoS One 2018;13: e0200940. doi:10.1371/journal.pone.0200940

Siebers M, Rohr T, Ventura M, Schütz V, Thies S, Kovacic F, Jaeger K-E, Berg M, Dörmann P, Schulz, M. Disruption of microbial community composition and identification of plant growth promoting microorganisms after exposure of soil to rapeseed-derived glucosinolates. PLoS One 2018;13: e0200160. doi:10.1371/journal.pone.0200160

Klein A, Loeschcke A, Jaeger K-E, Pietruszka J. Neue bioaktive Naturstoff-Derivate, Nachhaltige Produktion von Prodigiosinderivaten mittels Mutasynthese. GIT Labor-Fachzeitschrift. 2018 4: 41–43.

Klein AS, Brass HUC, Klebl D, Classen T, Loeschcke A, Drepper T, Sievers S, Jaeger K-E, Pietruszka J. Preparation of cyclic prodiginines by mutasynthesis in Pseudomonas putida KT2440. ChemBioChem 19: 1545–1552.doi:10.1002/cbic.201800154

Coscolín C, Martínez-Martínez M, Chow J, Bargiela R, García-Moyano A, Bjerga G, Bollinger A, Stokke R, Steen I, Golyshina O, Yakimov M, Jaeger K-E, Yakunin A, Streit W, Golyshin P, Ferrer M. Relationships between substrate promiscuity and chiral selectivity of esterases from phylogenetically and environmentally diverse microorganisms. Catalysts. 2018 8: 10. doi:10.3390/catal8010010

Martínez-Martínez M, Coscolín C, Santiago G, Chow J, Stogios PJ, Bargiela R, Gertler C, Navarro-Fernández J, Bollinger A, Thies S, Méndez-García C, Popovic A, Brown G, Chernikova TN, García-Moyano A, Bjerga GEK, Pérez-García P, Hai T, Del Pozo M V., Stokke R, Steen IH, Cui H, Xu X, Nocek BP, Alcaide M, Distaso M, Mesa V, Peláez AI, Sánchez J, Buchholz PCF, Pleiss J, Fernández-Guerra A, Glöckner FO, Golyshina O V., Yakimov MM, Savchenko A, Jaeger K-E, Yakunin AF, Streit WR, Golyshin PN, Guallar V, Ferrer M, The Inmare Consortium. Determinants and prediction of esterase substrate promiscuity patterns. ACS Chem Biol. 2018 13: 225–234. doi:10.1021/acschembio.7b00996



Loeschcke A, Dienst D, Wewer V, Hage-Hülsmann J, Dietsch M, Kranz-Finger S, Hüren V, Metzger S, Urlacher VB, Gigolashvili T, Kopriva S, Axmann IM, Drepper T, Jaeger K-E. The photosynthetic bacteria Rhodobacter capsulatus and Synechocystis sp. PCC 6803 as new hosts for cyclic plant triterpene biosynthesis. PLoS One. 2017 12: e0189816. doi:10.1371/journal.pone.0189816

Domröse A, Weihmann R, Thies S, Jaeger K-E, Drepper T, Loeschcke A. Rapid generation of recombinant Pseudomonas putida secondary metabolite producers using yTREX. Synth Syst Biotechnol. 2017 2: 310–319. doi:10.1016/j.synbio.2017.11.001

Klein AS, Domröse A, Bongen P, Brass HUC, Classen T, Loeschcke A, Drepper T, Laraia L, Sievers S, Jaeger K-E, Pietruszka J. New prodigiosin derivatives obtained by mutasynthesis in Pseudomonas putida. ACS Synth Biol. 2017 6: 1757–1765. doi:10.1021/acssynbio.7b00099

Poehlein A, Daniel R, Thürmer A, Bollinger A, Thies S, Katzke N, Jaeger K-E. First insights into the genome sequence of Pseudomonas oleovorans DSM 1045. Genome Announc. 2017 5: e00774-17. doi:10.1128/genomeA.00774-17

Tiso T, Thies S, Müller M, Tsvetanova L, Carraresi L, Bröring S, Jaeger K-E, Blank LM. Rhamnolipids: production, performance, and application. In: Lee SY, editor. Consequences of Microbial Interactions with Hydrocarbons, Oils, and Lipids: Production of Fuels and Chemicals. Cham: Springer International Publishing 2017. pp. 1–37. doi:10.1007/978-3-319-31421-1_388-1

Katzke N, Knapp A, Loeschcke A, Drepper T, Jaeger K-E. Novel tools for the functional expression of metagenomic DNA. In: Streit WR, Daniel R, editors. Metagenomics-methods and protocols. 2nd ed. New York: Springer New York 2017. pp. 159–196. doi:10.1007/978-1-4939-6691-2_10



Binder D, Probst C, Grünberger A, Hilgers F, Loeschcke A, Jaeger K-E, Kohlheyer D, Drepper T. Comparative single-cell analysis of different E. coli expression systems during microfluidic cultivation. PLoS One. 2016 11: e0160711. doi:10.1371/journal.pone.0160711

Binder D, Frohwitter J, Mahr R, Bier C, Grünberger A, Loeschcke A, Peters-Wendisch P, Kohlheyer D, Pietruszka J, Frunzke J, Jaeger K-E, Wendisch VF, Drepper T. Light-controlled cell factories: employing photocaged isopropyl-β-d-thiogalactopyranoside for light-mediated optimization of lac promoter-based gene expression and (+)-valencene biosynthesis in Corynebacterium glutamicum. Appl Environ Microbiol. 2016 82: 6141–6149. doi:10.1128/AEM.01457-16

Binder D, Bier C, Grünberger A, Drobietz D, Hage-Hülsmann J, Wandrey G, Büchs J, Kohlheyer D, Loeschcke A, Wiechert W, Jaeger K-E, Pietruszka J, Drepper T. Photocaged arabinose: a novel optogenetic switch for rapid and gradual control of microbial gene expression. Chembiochem. 2016 17: 296–9. doi:10.1002/cbic.201500609

Thies S, Rausch SC, Kovacic F, Schmidt-Thaler A, Wilhelm S, Rosenau F, Daniel R, Streit W, Pietruszka J, Jaeger K-E. Metagenomic discovery of novel enzymes and biosurfactants in a slaughterhouse biofilm microbial community. Sci Rep. 2016 6: 27035. doi:10.1038/srep27035



Domröse A, Klein AS, Hage-Hülsmann J, Thies S, Svensson V, Classen T, Pietruszka J, Jaeger K-E, Drepper T, Loeschcke A. Efficient recombinant production of prodigiosin in Pseudomonas putida. Front Microbiol. Frontiers 2015 6: 972. doi:10.3389/fmicb.2015.00972

Binder D, Probst C, Bier C, Loeschcke A, Grünberger A. Lichtgesteuerte Genexpression auf Einzelzellebene. BIOspektrum 2015 21: 612–615. doi:10.1007/s12268-015-0622-7

Loeschcke A, Thies S. Pseudomonas putida-a versatile host for the production of natural products. Appl Microbiol Biotechnol. 2015 99: 6197–214. doi:10.1007/s00253-015-6745-4



Liebl W, Angelov A, Juergensen J, Chow J, Loeschcke A, Drepper T, Classen T, Pietruszka J, Ehrenreich A, Streit WR, Jaeger K-E. Alternative hosts for functional (meta)genome analysis. Appl Microbiol Biotechnol. 2014 98: 8099–109. doi:10.1007/s00253-014-5961-7

Kaschner M, Loeschcke A, Krause J, Minh BQ, Heck A, Endres S, Svensson V, Wirtz A, von Haeseler A, Jaeger K-E, Drepper T, Krauss U. Discovery of the first light-dependent protochlorophyllide oxidoreductase in anoxygenic phototrophic bacteria. Mol Microbiol. 2014 doi:10.1111/mmi.12719

Binder D, Grünberger A, Loeschcke A, Probst C, Bier C, Pietruszka J, Wiechert W, Kohlheyer D, Jaeger K-E, Drepper T. Light-responsive control of bacterial gene expression: precise triggering of the lac promoter activity using photocaged IPTG. Integr Biol (Camb). 2014 6: 755–65. doi:10.1039/c4ib00027g

Thies S, Santiago-Schübel B, Kovačić F, Rosenau F, Hausmann R, Jaeger K-E. Heterologous production of the lipopeptide biosurfactant serrawettin W1 in Escherichia coli. J Biotechnol. 2014 181: 27–30. doi:10.1016/j.jbiotec.2014.03.037



Loeschcke A, Markert A, Wilhelm S, Wirtz A, Rosenau F, Jaeger K-E, Drepper T. TREX: a universal tool for the transfer and expression of biosynthetic pathways in bacteria. ACS Synth Biol. 2013 2: 22–33. doi:10.1021/sb3000657


Troeschel SC, Thies S, Link O, Real CI, Knops K, Wilhelm S, Rosenau F, Jaeger K-E. Novel broad host range shuttle vectors for expression in Escherichia coli, Bacillus subtilis and Pseudomonas putida. J Biotechnol. 2012 161: 71–9. doi:10.1016/j.jbiotec.2012.02.020

Arvani S, Markert A, Loeschcke A, Jaeger K-E, Drepper T. A T7 RNA polymerase-based toolkit for the concerted expression of clustered genes. J Biotechnol. 2012159: 162–71. doi:10.1016/j.jbiotec.2012.01.004