Zum Inhalt springenZur Suche springen

Publications (August 2021)

2021 Hage-Hülsmann J, Klaus O, Linke K, Troost K, Gora L, Hilgers F, Wirtz A, Santiago-Schübel B, Loeschcke A, Jaeger K-E,  Drepper, T. Production of C20, C30 and C40 terpenes in the engineered phototrophic bacterium Rhodobacter capsulatus. J Biotechnol. Elsevier; 2021;338: 20–30. doi:10.1016/j.jbiotec.2021.07.002
  Bitzenhofer NL, Kruse L, Thies S, Wynands B, Lechtenberg T, Rönitz J, Kozaeva E, Wirth NT, Eberlein C, Jaeger K-E, Nikel PI, Heipieper HJ, Wierckx N, Loeschcke A. Towards robust Pseudomonas cell factories to harbour novel biosynthetic pathways. Essays Biochem. 2021;65: 319–336. doi:10.1042/EBC20200173

Hilgers F, Habash SS, Loeschcke A, Sebastian Ackermann Y, Neumann S, Heck A, Klaus O, Hage-Hülsmann J, Grundler FMW, Jaeger K-E, Schleker ASS, Drepper T. Heterologous production of β-caryophyllene and evaluation of its activity against plant pathogenic fungi. Microorganisms. 2021;9: 168. doi:10.3390/microorganisms9010168


Höppner A, Bollinger A, Kobus S, Thies S, Coscolín C, Ferrer M, Jaeger K-E, Smits SHJ. Crystal structures of a novel family IV esterase in free and substrate‐bound form. FEBS J; 2021;. 288: 3570–3584. doi:10.1111/febs.15680


Chernomor O, Peters L, Schneidewind J, Loeschcke A, Knieps-Grünhagen E, Schmitz F, , von Lieres E, Kutta R J, Svensson V, Jaeger K-E, Drepper T, von Haeseler A, Krauss, U. Complex Evolution of Light-Dependent Protochlorophyllide Oxidoreductases in Aerobic Anoxygenic Phototrophs: Origin, Phylogeny, and Function. Mol Biol Evol. 2021;38: 819–837. doi:10.1093/molbev/msaa234

2020 Molitor R. Blog entry on expedition SO279. In: Hotmic – Transport and Fate of Marine Microplastics Blog. 2020
  Habash SS, Könen PP, Loeschcke A, Wüst M, Jaeger K-E, Drepper T, Grundler FMW, Schleker ASS. The Plant Sesquiterpene Nootkatone Efficiently Reduces Heterodera schachtii Parasitism by Activating Plant Defense. Int J Mol Sci. Int J Mol Sci; 2020;21: 9627. doi:10.3390/ijms21249627


Tiso T, Ihling N, Kubicki S, Biselli A, Schonhoff A, Bator I, Loeschcke A, Zapp P, Jupke A, Jaeger K-E, Büchs J, Blank LM. Integration of genetic and process engineering for optimized rhamnolipid production using Pseudomonas putida. Front Bioeng Biotechnol. 2020;8: 976. doi:10.3389/fbioe.2020.00976

Kubicki S, Bator I, Jankowski S, Schipper K, Tiso T, Feldbrügge M, Blank LM, Thies S, Jaeger K-E. A straightforward assay for screening and quantification of biosurfactants in microbial culture supernatants. Front Bioeng Biotechnol. 2020;8: 958. doi:10.3389/fbioe.2020.00958

Lee J, Hilgers F, Loeschke A, Jaeger K-E, Feldbrügge M. Ustilago maydis serves as a novel production host for the synthesis of plant and fungal sesquiterpenoids. Front Microbiol. 2020;11: 1655. doi:10.3389/fmicb.2020.01655

Loeschcke A, Thies S. Engineering of natural product biosynthesis in Pseudomonas putida.
Curr Opin Biotechnol. 2020;65: 213–224. doi:10.1016/j.copbio.2020.03.007

Bollinger A, Molitor R, Thies S, Koch R, Coscolín C, Ferrer M, Jaeger K-E. Identification of organic solvent tolerant carboxylic ester hydrolases for organic synthesis. Appl Environ Microbiol. 2020; doi:10.1128/AEM.00106-20

Hage-Hülsmann J, Metzger S, Wewer V, Buechel F, Troost K, Thies S, Loeschcke A., Jaeger K-E, Drepper T. Biosynthesis of cycloartenol by expression of plant and bacterial oxidosqualene cyclases in engineered Rhodobacter capsulatus.; J Biotechnol X. 2019;4: 100014. doi:10.1016/j.btecx.2020.100014

Bollinger A, Thies S, Knieps-Grünhagen E, Gertzen C, Kobus S, Höppner A, Ferrer M, Gohlke H, Smits SH, Jaeger K-E. A novel polyester hydrolase from the marine bacterium Pseudomonas aestusnigri -structural and functional insights. Front Microbiol. 2020;11: 114. doi:10.3389/FMICB.2020.00114

Molitor R, Bollinger A, Kubicki S, Loeschcke A, Jaeger K-E, Thies S. Agar plate-based screening methods for the identification of polyester hydrolysis by Pseudomonas species. Microb Biotechnol. 2020;13: 274–284. doi:10.1111/1751-7915.13418

Weihmann R, Domröse A, Drepper T, Jaeger K-E, Loeschcke A. Protocols for yTREX/Tn5-based gene cluster expression in Pseudomonas putida. Microb Biotechnol. 2020;13: 250–262. doi:10.1111/1751-7915.13402

Bollinger A, Thies S, Katzke N, Jaeger K-E. The biotechnological potential of marine bacteria in the novel lineage of Pseudomonas pertucinogena. Microb Biotechnol. 2020;13: 19–31. doi:10.1111/1751-7915.13288


Novel carboxyesterhydrolases. EP3572523A1, 2019. Available: patents.google.com/patent/EP3572523A1

Troost K, Loeschcke A, Hilgers F, Özgür AY, Weber TM, Santiago-Schübel B, Svensson V, Hage-Hülsmann J, Habash S, Grundler F, Schleker S, Jaeger K-E, Drepper T. Engineered Rhodobacter capsulatus as a phototrophic platform organism for the synthesis of plant sesquiterpenoids. Front Microbiol. 2019;10: 1998. doi:10.3389/FMICB.2019.01998

Otto M, Wynands B, Drepper T, Jaeger K-E, Thies S, Loeschcke A, Blank LM, Wierckx N. Targeting 16S rDNA for Stable Recombinant Gene Expression in Pseudomonas. ACS Synth Biol. 2019; acssynbio.9b00195. doi:10.1021/acssynbio.9b00195

Kubicki S, Bollinger A, Katzke N, Jaeger K-E, Loeschcke A, Thies S. Marine Biosurfactants: Biosynthesis, Structural Diversity and Biotechnological Applications. Mar Drugs. 2019;17: 408. doi:10.3390/md17070408

Domröse A, Hage-Hülsmann J, Thies S, Weihmann R, Kruse L, Otto M, Wierckx N, Jaeger K‑E, Drepper T, Loeschcke A. Pseudomonas putida rDNA is a favored site for the expression of biosynthetic genes. Sci Reports 2019;9: 7028. doi:10.1038/s41598-019-43405-1

Peters L, Weidenfeld I, Klemm U, Loeschcke A, Weihmann R, Jaeger K-E, Drepper T, Ntziachristos V, Stiel A C. Phototrophic purple bacteria as optoacoustic in vivo reporters of macrophage activity. Nat Commun. 2019;10: 1191. doi:10.1038/s41467-019-09081-5







Coscolín C, Katzke N, García-Moyano A, Navarro-Fernández J., Almendral D., a Martínez-Martínez M., Bollinger A., Bargiela R., Gerler C., Chernikova T., Rojo D., Barbas C, Hai T, Golyshina O, Koch R, Yakimov M, Bjerga G, Golyshin P, Jaeger K-E, and Ferrer M, the INMARE Consortium. Bioprospecting reveals class III ω-transaminases converting bulky ketones and environmentally relevant polyamines. Appl Environ Microbiol. American Society for Microbiology; 2018; AEM.02404-18. doi:10.1128/AEM.02404-18

Coscolín C, Bargiela R, Martínez-Martínez M, Alonso S, Bollinger A, Thies S, Chernikova TN, Hai T, Golyshina OV, Jaeger K-E, Yakimov MM, Golyshin PN, Ferrer M. Hydrocarbon-Degrading Microbes as Sources of New Biocatalysts. In: McGenity TJ, editor. Taxonomy, Genomics and Ecophysiology of Hydrocarbon-Degrading Microbes. Cham: Springer International Publishing; 2018. pp. 353-373. doi: 10.1007/978-3-030-14796-9_13

Kubicki S, Weihmann R, Bator I, Domröse A, Loeschcke A, Drepper T, Tiso T, Blank L, Jaeger K-E, Thies S. Konstruktion von Pseudomonas putida -Stämmen zur heterologen Produktion von Rhamnolipiden. Chemie Ing Tech. Wiley-Blackwell; 2018;90: 1282–1282. doi:10.1002/cite.201855328

Klein AS, Brass HUC, Klebl DP, Classen T, Loeschcke A, Drepper T, Sievers S, Jaeger K-., Pietruszka J. Cover Feature: Preparation of Cyclic Prodiginines by Mutasynthesis in Pseudomonas putida KT2440 (ChemBioChem 14/2018). ChemBioChem. Wiley-Blackwell; 2018;19: 1462–1462. doi:10.1002/cbic.201800328

Hage-Hülsmann J, Grünberger A, Thies S, Santiago-Schübel B, Klein AS, Pietruszka J, Binder D, Hilgers F, Domröse A, Drepper T, Kohlheyer D, Jaeger K-E, Loeschcke A. Natural biocide cocktails: Combinatorial antibiotic effects of prodigiosin and biosurfactants. PLoS One 2018;13: e0200940. doi:10.1371/journal.pone.0200940

Siebers M, Rohr T, Ventura M, Schütz V, Thies S, Kovacic F, Jaeger K-E, Berg M, Dörmann P, Schulz, M. Disruption of microbial community composition and identification of plant growth promoting microorganisms after exposure of soil to rapeseed-derived glucosinolates. PLoS One 2018;13: e0200160. doi:10.1371/journal.pone.0200160

Klein A, Loeschcke A, Jaeger K-E, Pietruszka J. Neue bioaktive Naturstoff-Derivate, Nachhaltige Produktion von Prodigiosinderivaten mittels Mutasynthese. GIT Labor-Fachzeitschrift. 2018 4: 41–43.

Klein AS, Brass HUC, Klebl D, Classen T, Loeschcke A, Drepper T, Sievers S, Jaeger K-E, Pietruszka J. Preparation of cyclic prodiginines by mutasynthesis in Pseudomonas putida KT2440. ChemBioChem 19: 1545–1552.doi:10.1002/cbic.201800154

Coscolín C, Martínez-Martínez M, Chow J, Bargiela R, García-Moyano A, Bjerga G, Bollinger A, Stokke R, Steen I, Golyshina O, Yakimov M, Jaeger K-E, Yakunin A, Streit W, Golyshin P, Ferrer M. Relationships between substrate promiscuity and chiral selectivity of esterases from phylogenetically and environmentally diverse microorganisms. Catalysts. 2018 8: 10. doi:10.3390/catal8010010

Martínez-Martínez M, Coscolín C, Santiago G, Chow J, Stogios PJ, Bargiela R, Gertler C, Navarro-Fernández J, Bollinger A, Thies S, Méndez-García C, Popovic A, Brown G, Chernikova TN, García-Moyano A, Bjerga GEK, Pérez-García P, Hai T, Del Pozo M V., Stokke R, Steen IH, Cui H, Xu X, Nocek BP, Alcaide M, Distaso M, Mesa V, Peláez AI, Sánchez J, Buchholz PCF, Pleiss J, Fernández-Guerra A, Glöckner FO, Golyshina O V., Yakimov MM, Savchenko A, Jaeger K-E, Yakunin AF, Streit WR, Golyshin PN, Guallar V, Ferrer M, The Inmare Consortium. Determinants and prediction of esterase substrate promiscuity patterns. ACS Chem Biol. 2018 13: 225–234. doi:10.1021/acschembio.7b00996



Loeschcke A, Dienst D, Wewer V, Hage-Hülsmann J, Dietsch M, Kranz-Finger S, Hüren V, Metzger S, Urlacher VB, Gigolashvili T, Kopriva S, Axmann IM, Drepper T, Jaeger K-E. The photosynthetic bacteria Rhodobacter capsulatus and Synechocystis sp. PCC 6803 as new hosts for cyclic plant triterpene biosynthesis. PLoS One. 2017 12: e0189816. doi:10.1371/journal.pone.0189816

Domröse A, Weihmann R, Thies S, Jaeger K-E, Drepper T, Loeschcke A. Rapid generation of recombinant Pseudomonas putida secondary metabolite producers using yTREX. Synth Syst Biotechnol. 2017 2: 310–319. doi:10.1016/j.synbio.2017.11.001

Klein AS, Domröse A, Bongen P, Brass HUC, Classen T, Loeschcke A, Drepper T, Laraia L, Sievers S, Jaeger K-E, Pietruszka J. New prodigiosin derivatives obtained by mutasynthesis in Pseudomonas putida. ACS Synth Biol. 2017 6: 1757–1765. doi:10.1021/acssynbio.7b00099

Poehlein A, Daniel R, Thürmer A, Bollinger A, Thies S, Katzke N, Jaeger K-E. First insights into the genome sequence of Pseudomonas oleovorans DSM 1045. Genome Announc. 2017 5: e00774-17. doi:10.1128/genomeA.00774-17

Tiso T, Thies S, Müller M, Tsvetanova L, Carraresi L, Bröring S, Jaeger K-E, Blank LM. Rhamnolipids: production, performance, and application. In: Lee SY, editor. Consequences of Microbial Interactions with Hydrocarbons, Oils, and Lipids: Production of Fuels and Chemicals. Cham: Springer International Publishing 2017. pp. 1–37. doi:10.1007/978-3-319-31421-1_388-1

Katzke N, Knapp A, Loeschcke A, Drepper T, Jaeger K-E. Novel tools for the functional expression of metagenomic DNA. In: Streit WR, Daniel R, editors. Metagenomics-methods and protocols. 2nd ed. New York: Springer New York 2017. pp. 159–196. doi:10.1007/978-1-4939-6691-2_10



Binder D, Probst C, Grünberger A, Hilgers F, Loeschcke A, Jaeger K-E, Kohlheyer D, Drepper T. Comparative single-cell analysis of different E. coli expression systems during microfluidic cultivation. PLoS One. 2016 11: e0160711. doi:10.1371/journal.pone.0160711

Binder D, Frohwitter J, Mahr R, Bier C, Grünberger A, Loeschcke A, Peters-Wendisch P, Kohlheyer D, Pietruszka J, Frunzke J, Jaeger K-E, Wendisch VF, Drepper T. Light-controlled cell factories: employing photocaged isopropyl-β-d-thiogalactopyranoside for light-mediated optimization of lac promoter-based gene expression and (+)-valencene biosynthesis in Corynebacterium glutamicum. Appl Environ Microbiol. 2016 82: 6141–6149. doi:10.1128/AEM.01457-16

Binder D, Bier C, Grünberger A, Drobietz D, Hage-Hülsmann J, Wandrey G, Büchs J, Kohlheyer D, Loeschcke A, Wiechert W, Jaeger K-E, Pietruszka J, Drepper T. Photocaged arabinose: a novel optogenetic switch for rapid and gradual control of microbial gene expression. Chembiochem. 2016 17: 296–9. doi:10.1002/cbic.201500609

Thies S, Rausch SC, Kovacic F, Schmidt-Thaler A, Wilhelm S, Rosenau F, Daniel R, Streit W, Pietruszka J, Jaeger K-E. Metagenomic discovery of novel enzymes and biosurfactants in a slaughterhouse biofilm microbial community. Sci Rep. 2016 6: 27035. doi:10.1038/srep27035



Domröse A, Klein AS, Hage-Hülsmann J, Thies S, Svensson V, Classen T, Pietruszka J, Jaeger K-E, Drepper T, Loeschcke A. Efficient recombinant production of prodigiosin in Pseudomonas putida. Front Microbiol. Frontiers 2015 6: 972. doi:10.3389/fmicb.2015.00972

Binder D, Probst C, Bier C, Loeschcke A, Grünberger A. Lichtgesteuerte Genexpression auf Einzelzellebene. BIOspektrum 2015 21: 612–615. doi:10.1007/s12268-015-0622-7

Loeschcke A, Thies S. Pseudomonas putida-a versatile host for the production of natural products. Appl Microbiol Biotechnol. 2015 99: 6197–214. doi:10.1007/s00253-015-6745-4



Liebl W, Angelov A, Juergensen J, Chow J, Loeschcke A, Drepper T, Classen T, Pietruszka J, Ehrenreich A, Streit WR, Jaeger K-E. Alternative hosts for functional (meta)genome analysis. Appl Microbiol Biotechnol. 2014 98: 8099–109. doi:10.1007/s00253-014-5961-7

Kaschner M, Loeschcke A, Krause J, Minh BQ, Heck A, Endres S, Svensson V, Wirtz A, von Haeseler A, Jaeger K-E, Drepper T, Krauss U. Discovery of the first light-dependent protochlorophyllide oxidoreductase in anoxygenic phototrophic bacteria. Mol Microbiol. 2014 doi:10.1111/mmi.12719

Binder D, Grünberger A, Loeschcke A, Probst C, Bier C, Pietruszka J, Wiechert W, Kohlheyer D, Jaeger K-E, Drepper T. Light-responsive control of bacterial gene expression: precise triggering of the lac promoter activity using photocaged IPTG. Integr Biol (Camb). 2014 6: 755–65. doi:10.1039/c4ib00027g

Thies S, Santiago-Schübel B, Kovačić F, Rosenau F, Hausmann R, Jaeger K-E. Heterologous production of the lipopeptide biosurfactant serrawettin W1 in Escherichia coli. J Biotechnol. 2014 181: 27–30. doi:10.1016/j.jbiotec.2014.03.037



Loeschcke A, Markert A, Wilhelm S, Wirtz A, Rosenau F, Jaeger K-E, Drepper T. TREX: a universal tool for the transfer and expression of biosynthetic pathways in bacteria. ACS Synth Biol. 2013 2: 22–33. doi:10.1021/sb3000657


Troeschel SC, Thies S, Link O, Real CI, Knops K, Wilhelm S, Rosenau F, Jaeger K-E. Novel broad host range shuttle vectors for expression in Escherichia coli, Bacillus subtilis and Pseudomonas putida. J Biotechnol. 2012 161: 71–9. doi:10.1016/j.jbiotec.2012.02.020

Arvani S, Markert A, Loeschcke A, Jaeger K-E, Drepper T. A T7 RNA polymerase-based toolkit for the concerted expression of clustered genes. J Biotechnol. 2012159: 162–71. doi:10.1016/j.jbiotec.2012.01.004